Simão Fabrício, Matté Aline, Breier Ana Carolina, Kreutz Fernando, Trindade Vera Maria Treis, Netto Carlos Alexandre, Salbego Christianne G
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Porto Alegre, RS, Brazil.
Neurol Res. 2013 Jan;35(1):59-64. doi: 10.1179/1743132812Y.0000000116.
The present study was undertaken to evaluate whether resveratrol (RSV) modulates membrane lipid composition, as well as on ganglioside profile in ischemia/reperfusion injury.
Global cerebral ischemia was induced by four-vessel occlusion for 10 minutes. RSV (30 mg/kg) or vehicle was intraperitoneally administered to rats 7 days prior to ischemia. Brain structures were homogenized with chloroform/methanol for ganglioside, phospholipids, and cholesterol levels.
RSV significantly prevented the reduction in the total content of gangliosides, phospholipids, and cholesterol in hippocampi and cerebral cortex induced by global cerebral ischemia. Although ischemia/reperfusion decreased ganglioside content, the ganglioside profiles were apparently not modified.
Our experiments suggest that lipid metabolism is important for development of ischemic damage and indicate that RSV treatment 7 days prior to ischemia may prevent membrane lipid loss.
本研究旨在评估白藜芦醇（RSV）是否能调节缺血/再灌注损伤中的膜脂质成分以及神经节苷脂谱。
通过四动脉闭塞10分钟诱导全脑缺血。在缺血前7天，将RSV（30毫克/千克）或赋形剂腹腔注射给大鼠。用氯仿/甲醇将脑结构匀浆以测定神经节苷脂、磷脂和胆固醇水平。
RSV显著预防了全脑缺血诱导的海马体和大脑皮质中神经节苷脂、磷脂和胆固醇总含量的降低。尽管缺血/再灌注降低了神经节苷脂含量，但神经节苷脂谱显然未改变。
我们的实验表明脂质代谢对缺血性损伤的发展很重要，并表明缺血前7天进行RSV治疗可能预防膜脂质损失。
