多聚核糖体在哺乳动物处理体（P 体）附近的结构组织。
Structural organization of the polysomes adjacent to mammalian processing bodies (P-bodies).
机构信息
Université de Rennes 1, UMR, CNRS 6290 IGDR, Translation and Folding Team, Campus de Beaulieu, Rennes Cedex, France.
出版信息
RNA Biol. 2013 Feb;10(2):314-20. doi: 10.4161/rna.23342. Epub 2013 Jan 16.
Abstract
A finely tuned balance of translation, storage and decay of mRNAs (mRNAs) is important for the regulation of gene expression. In eukaryotic cells, this takes place in dynamic cytoplasmic RNA-protein granules termed Processing bodies (P-bodies). In this study, by using immunoelectron tomography, 3D modeling and template matching, we analyze the size and the organization of the polysomes in the vicinity of human P-bodies. Our results show the presence of several polysomes that are compatible with a translational activity around P-bodies. Therefore, movement of mRNAs between polysomes and P-bodies can take place when the two compartments are in close contact. The presence of initiation factors in the proximity of P-bodies also suggests that translation of mRNAs can resume at the periphery of these granules.
摘要
mRNA（信使 RNA）的翻译、储存和降解的精细平衡对于基因表达的调控非常重要。在真核细胞中，这种平衡发生在称为处理体（P 体）的动态细胞质 RNA-蛋白质颗粒中。在这项研究中，我们通过免疫电子断层扫描、三维建模和模板匹配，分析了人 P 体附近多核糖体的大小和组织。我们的结果表明，存在几种多核糖体，这与 P 体周围的翻译活性相容。因此，当这两个隔室紧密接触时，mRNA 可以在多核糖体和 P 体之间移动。P 体附近存在起始因子也表明这些颗粒的外围可以重新开始翻译 mRNA。
相似文献
1
Structural organization of the polysomes adjacent to mammalian processing bodies (P-bodies).多聚核糖体在哺乳动物处理体（P 体）附近的结构组织。
RNA Biol. 2013 Feb;10(2):314-20. doi: 10.4161/rna.23342. Epub 2013 Jan 16.
2
Polysomes, P bodies and stress granules: states and fates of eukaryotic mRNAs.多核糖体、P小体与应激颗粒：真核生物mRNA的状态与命运
Curr Opin Cell Biol. 2009 Jun;21(3):403-8. doi: 10.1016/j.ceb.2009.03.005. Epub 2009 Apr 23.
3
Movement of eukaryotic mRNAs between polysomes and cytoplasmic processing bodies.真核生物信使核糖核酸在多核糖体与细胞质加工小体之间的移动
Science. 2005 Oct 21;310(5747):486-9. doi: 10.1126/science.1115791. Epub 2005 Sep 1.
4
Translational Control of Canonical and Non-Canonical Translation Initiation Factors at the Sea Urchin Egg to Embryo Transition.海胆卵到胚胎转变过程中经典和非经典翻译起始因子的翻译调控。
Int J Mol Sci. 2019 Feb 1;20(3):626. doi: 10.3390/ijms20030626.
5
Probing the closed-loop model of mRNA translation in living cells.探究活细胞中mRNA翻译的闭环模型。
RNA Biol. 2015;12(3):248-54. doi: 10.1080/15476286.2015.1017242.
6
Stress granules and processing bodies are dynamically linked sites of mRNP remodeling.应激颗粒和加工小体是mRNA核糖核蛋白重塑的动态连接位点。
J Cell Biol. 2005 Jun 20;169(6):871-84. doi: 10.1083/jcb.200502088.
7
Polysomes of Trypanosoma brucei: Association with Initiation Factors and RNA-Binding Proteins.布氏锥虫的多核糖体：与起始因子和RNA结合蛋白的关联
PLoS One. 2015 Aug 19;10(8):e0135973. doi: 10.1371/journal.pone.0135973. eCollection 2015.
8
Accumulation of polyadenylated mRNA, Pab1p, eIF4E, and eIF4G with P-bodies in Saccharomyces cerevisiae.酿酒酵母中多聚腺苷酸化mRNA、Pab1p、eIF4E和eIF4G与P小体的积累。
Mol Biol Cell. 2007 Jul;18(7):2592-602. doi: 10.1091/mbc.e06-12-1149. Epub 2007 May 2.
9
Translational control of mRNAs by 3'-Untranslated region binding proteins.通过 3'-非翻译区结合蛋白对 mRNAs 的翻译调控。
BMB Rep. 2017 Apr;50(4):194-200. doi: 10.5483/bmbrep.2017.50.4.040.
10
P-bodies and stress granules: possible roles in the control of translation and mRNA degradation.P 体和应激颗粒：在翻译和 mRNA 降解控制中的可能作用。
Cold Spring Harb Perspect Biol. 2012 Sep 1;4(9):a012286. doi: 10.1101/cshperspect.a012286.
引用本文的文献
1
A proxitome-RNA-capture approach reveals that processing bodies repress coregulated hub genes.一种临近组学-RNA 捕获方法揭示了处理体抑制共调控枢纽基因。
Plant Cell. 2024 Feb 26;36(3):559-584. doi: 10.1093/plcell/koad288.
2
mRNA Targeting, Transport and Local Translation in Eukaryotic Cells: From the Classical View to a Diversity of New Concepts.真核细胞中的mRNA靶向、转运与局部翻译：从经典观点到多样的新概念
Mol Biol. 2021;55(4):507-537. doi: 10.1134/S0026893321030080. Epub 2021 May 30.
3
Metabolism and Functions of Inositol Pyrophosphates: Insights Gained from the Application of Synthetic Analogues.肌醇焦磷酸盐的代谢和功能：通过应用合成类似物获得的见解。
Molecules. 2020 Oct 2;25(19):4515. doi: 10.3390/molecules25194515.
4
InsP is a small-molecule regulator of NUDT3-mediated mRNA decapping and processing-body dynamics.InsP 是一种小分子调节剂，可调节 NUDT3 介导的 mRNA 去帽和处理体动力学。
Proc Natl Acad Sci U S A. 2020 Aug 11;117(32):19245-19253. doi: 10.1073/pnas.1922284117. Epub 2020 Jul 29.
5
mRNP granules. Assembly, function, and connections with disease.信使核糖核蛋白颗粒。组装、功能及其与疾病的关联。
RNA Biol. 2014;11(8):1019-30. doi: 10.4161/15476286.2014.972208.
6
Translation and silencing in RNA granules: a tale of sand grains.RNA 颗粒中的翻译和沉默：沙粒的故事。
Front Mol Neurosci. 2014 Jul 23;7:68. doi: 10.3389/fnmol.2014.00068. eCollection 2014.
7
Synaptic control of local translation: the plot thickens with new characters.突触对局部翻译的控制：新角色的加入使情节更加复杂。
Cell Mol Life Sci. 2014 Jun;71(12):2219-39. doi: 10.1007/s00018-013-1506-y. Epub 2013 Nov 10.
本文引用的文献
1
P-bodies and stress granules: possible roles in the control of translation and mRNA degradation.P 体和应激颗粒：在翻译和 mRNA 降解控制中的可能作用。
Cold Spring Harb Perspect Biol. 2012 Sep 1;4(9):a012286. doi: 10.1101/cshperspect.a012286.
2
The dual organization of P-bodies revealed by immunoelectron microscopy and electron tomography.免疫电子显微镜和电子断层扫描揭示的 P 体的双重组织。
J Mol Biol. 2012 Jun 29;420(1-2):17-28. doi: 10.1016/j.jmb.2012.03.027. Epub 2012 Apr 3.
3
Sequestration of highly expressed mRNAs in cytoplasmic granules, P-bodies, and stress granules enhances cell viability.细胞质颗粒、P 体和应激颗粒中高度表达的 mRNAs 的隔离增强了细胞活力。
PLoS Genet. 2012;8(2):e1002527. doi: 10.1371/journal.pgen.1002527. Epub 2012 Feb 23.
4
Reconsidering movement of eukaryotic mRNAs between polysomes and P bodies.重新考虑真核生物 mRNA 在多核糖体和 P 体之间的运动。
Mol Cell. 2011 Dec 9;44(5):745-58. doi: 10.1016/j.molcel.2011.09.019.
5
Visual proteomics.视觉蛋白质组学
Methods Enzymol. 2010;483:215-43. doi: 10.1016/S0076-6879(10)83011-3.
6
The three-dimensional organization of polyribosomes in intact human cells.完整的人细胞中多聚核糖体的三维结构。
Mol Cell. 2010 Aug 27;39(4):560-9. doi: 10.1016/j.molcel.2010.08.003.
7
The pioneer round of translation: features and functions.首轮翻译：特点和功能。
Cell. 2010 Aug 6;142(3):368-74. doi: 10.1016/j.cell.2010.07.022.
8
The mechanism of eukaryotic translation initiation and principles of its regulation.真核生物翻译起始的机制与调控原则。
Nat Rev Mol Cell Biol. 2010 Feb;11(2):113-27. doi: 10.1038/nrm2838.
9
Co-translational mRNA decay in Saccharomyces cerevisiae.酿酒酵母中的共翻译mRNA衰变
Nature. 2009 Sep 10;461(7261):225-9. doi: 10.1038/nature08265. Epub 2009 Aug 23.
10
Polysomes, P bodies and stress granules: states and fates of eukaryotic mRNAs.多核糖体、P小体与应激颗粒：真核生物mRNA的状态与命运
Curr Opin Cell Biol. 2009 Jun;21(3):403-8. doi: 10.1016/j.ceb.2009.03.005. Epub 2009 Apr 23.
Zotero 插件