Department of Applied Plant Sciences, Graduate School, Sangji University, Wonju, Korea.
Arch Pharm Res. 2013 Jan;36(1):51-60. doi: 10.1007/s12272-013-0015-8.
The flowers or leaves of Chrysanthemum boreale (Compositae) have been traditionally used as herb tea to reduce anxiety, insomnia, and stress. Sedative and anticonvulsant activities were evaluated in mice using pentobarbital-induced sleeping assay and pentylenetetrazole (PTZ)-induced convulsion assay. The flower extract exhibited more potent activities than the extracts of the leaves and stems, and chromatographic isolation yielded the five compounds acacetin, linarin, acacetin 7-O-β-D-glucopyranosyl-(1 → 2)[α-L-rhamnopyranosyl-(1 → 6)]-β-D-glucopyranoside, chlorogenic acid, and 3,5-di-O-caffeoylquinic acid. These compounds were simultaneously analyzed by HPLC, and the method was validated. The contents of linarin, which were shown to be most abundant in C. boreale, were observed in the order of leaf (11.93 mg/g) > flower (8.50 mg/g) > stem (5.60 mg/g). Linarin and its aglycone, acacetin, exhibited sedative and anticonvulsant activities in the present in vivo assays. It can be considered that linarin is one of the active compounds effective against anxiety, insomnia, and stress, with acacetin as its active moiety.
紫菀（菊科）的花或叶历来被用作草药茶，以减轻焦虑、失眠和压力。本文采用戊巴比妥钠诱导睡眠试验和戊四氮（PTZ）诱导惊厥试验，评价了紫菀花、叶和茎提取物的镇静和抗惊厥活性。花提取物的活性强于叶和茎提取物，色谱分离得到了 5 种化合物：木犀草素、芫花素、木犀草素 7-O-β-D-吡喃葡萄糖基（1 → 2）[α-L-鼠李吡喃糖基（1 → 6）]-β-D-吡喃葡萄糖苷、绿原酸和 3,5-二-O-咖啡酰奎宁酸。采用 HPLC 同时分析这些化合物，并对方法进行了验证。在紫菀中含量最高的芫花素的含量顺序为叶（11.93mg/g）>花（8.50mg/g）>茎（5.60mg/g）。在本体内试验中，芫花素及其苷元木犀草素表现出镇静和抗惊厥活性。可以认为，芫花素是一种有效治疗焦虑、失眠和压力的活性化合物，其活性部分是木犀草素。
