采用多重实时 PCR 快速同步检测 blaKPC 和 blaNDM。
Rapid and simultaneous detection of blaKPC and blaNDM by use of multiplex real-time PCR.
机构信息
Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
出版信息
J Clin Microbiol. 2013 Apr;51(4):1247-9. doi: 10.1128/JCM.03316-12. Epub 2013 Jan 16.
Abstract
The increasing incidence of carbapenem nonsusceptibility among clinically important species is of global concern. Identification of the molecular mechanisms underlying carbapenem nonsusceptibility is critical for epidemiological investigations. In this report, we describe a real-time PCR-based assay capable of simultaneously detecting blaKPC and blaNDM, two of the most important carbapenemases, directly from culture in less than 90 min. The assay was validated with blaKPC- and blaNDM-carrying clinical isolates and demonstrated 100% concordance with the Carba NP test.
摘要
临床重要菌种碳青霉烯类药物不敏感的发生率不断增加，引起了全球关注。确定碳青霉烯类药物不敏感的分子机制对于流行病学研究至关重要。在本报告中，我们描述了一种基于实时 PCR 的检测方法，能够直接从培养物中快速（不到 90 分钟）同时检测 blaKPC 和 blaNDM，这两种是最重要的碳青霉烯酶之一。该检测方法用携带 blaKPC 和 blaNDM 的临床分离株进行了验证，与 Carba NP 检测的一致性达到 100%。
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