Crivello Giulia, Orlandini Giuliana, Morena Angela Gala, Torchio Alessandro, Mattu Clara, Boffito Monica, Tzanov Tzanko, Ciardelli Gianluca
Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy.
Group of Molecular and Industrial Biotechnology, Department of Chemical Engineering, Universitat Politècnica de Catalunya, 08222 Terrassa, Spain.
Pharmaceutics. 2023 Jun 13;15(6):1717. doi: 10.3390/pharmaceutics15061717.
Chronic wounds (CWs) are a growing issue for the health care system. Their treatment requires a synergic approach to reduce both inflammation and the bacterial burden. In this work, a promising system for treating CWs was developed, comprising cobalt-lignin nanoparticles (NPs) embedded in a supramolecular (SM) hydrogel. First, NPs were obtained through cobalt reduction with phenolated lignin, and their antibacterial properties were tested against both Gram-negative and Gram-positive strains. The anti-inflammatory capacity of the NPs was proven through their ability to inhibit myeloperoxidase (MPO) and matrix metalloproteases (MMPs), which are enzymes involved in the inflammatory process and wound chronicity. Then, the NPs were loaded in an SM hydrogel based on a blend of α-cyclodextrin and custom-made poly(ether urethane)s. The nano-enabled hydrogel showed injectability, self-healing properties, and linear release of the loaded cargo. Moreover, the SM hydrogel's characteristics were optimized to absorb proteins when in contact with liquid, suggesting its capacity to uptake harmful enzymes from the wound exudate. These results render the developed multifunctional SM material an interesting candidate for the management of CWs.
慢性伤口（CWs）对医疗保健系统而言是一个日益严重的问题。其治疗需要一种协同方法来减轻炎症和细菌负荷。在这项工作中，开发了一种用于治疗慢性伤口的有前景的系统，该系统由嵌入超分子（SM）水凝胶中的钴-木质素纳米颗粒（NPs）组成。首先，通过用酚化木质素还原钴获得纳米颗粒，并测试了它们对革兰氏阴性菌和革兰氏阳性菌菌株的抗菌性能。纳米颗粒的抗炎能力通过其抑制髓过氧化物酶（MPO）和基质金属蛋白酶（MMPs）的能力得到证明，这两种酶参与炎症过程和伤口慢性化。然后，将纳米颗粒负载到基于α-环糊精和定制聚（醚聚氨酯）共混物的超分子水凝胶中。这种纳米增强水凝胶具有可注射性、自愈特性以及负载物质的线性释放特性。此外，超分子水凝胶的特性经过优化，使其在与液体接触时能够吸收蛋白质，这表明它有能力从伤口渗出液中摄取有害酶。这些结果使得所开发的多功能超分子材料成为治疗慢性伤口的一个有吸引力的候选材料。
