Wise Laboratory of Environmental and Genetic Toxicology, University of Southern Maine, 96 Falmouth St., P.O. Box 9300, Portland, ME 04104, USA.
Aquat Toxicol. 2010 Apr 1;97(1):34-41. doi: 10.1016/j.aquatox.2009.11.016. Epub 2009 Dec 2.
Nanoparticles are being widely investigated for a range of applications due to their unique physical properties. For example, silver nanoparticles are used in commercial products for their antibacterial and antifungal properties. Some of these products are likely to result in silver nanoparticles reaching the aquatic environment. As such, nanoparticles pose a health concern for humans and aquatic species. We used a medaka (Oryzias latipes) cell line to investigate the cytotoxicity and genotoxicity of 30nm diameter silver nanospheres. Treatments of 0.05, 0.3, 0.5, 3 and 5microg/cm(2) induced 80, 45.7, 24.3, 1 and 0.1% survival, respectively, in a colony forming assay. Silver nanoparticles also induced chromosomal aberrations and aneuploidy. Treatments of 0, 0.05, 0.1 and 0.3microg/cm(2) induced damage in 8, 10.8, 16 and 15.8% of metaphases and 10.8, 15.6, 24 and 24 total aberrations in 100 metaphases, respectively. These data show that silver nanoparticles are cytotoxic and genotoxic to fish cells.
纳米颗粒由于其独特的物理性质而被广泛应用于各种领域。例如，银纳米颗粒因其具有抗菌和抗真菌特性而被用于商业产品中。其中一些产品可能会导致银纳米颗粒进入水生环境。因此，纳米颗粒对人类和水生生物的健康构成了威胁。我们使用了一种斑马鱼（Oryzias latipes）细胞系来研究 30nm 直径的银纳米球的细胞毒性和遗传毒性。在集落形成测定中，浓度为 0.05、0.3、0.5、3 和 5μg/cm(2)的处理分别导致 80%、45.7%、24.3%、1%和 0.1%的细胞存活。银纳米颗粒还诱导了染色体畸变和非整倍体。在 0、0.05、0.1 和 0.3μg/cm(2)的处理下，分别有 8%、10.8%、16%和 15.8%的中期分裂细胞受到损伤，在 100 个中期分裂细胞中，分别有 10.8%、15.6%、24%和 24%的总染色体畸变。这些数据表明，银纳米颗粒对鱼类细胞具有细胞毒性和遗传毒性。
