一种生物物理 miRNA-mRNA 相互作用模型推断出规范和非规范靶标。
A biophysical miRNA-mRNA interaction model infers canonical and noncanonical targets.
机构信息
Biozentrum, University of Basel, Basel, Switzerland.
出版信息
Nat Methods. 2013 Mar;10(3):253-5. doi: 10.1038/nmeth.2341. Epub 2013 Jan 20.
PMID:23334102
Abstract
We introduce a biophysical model of miRNA-target interaction and infer its parameters from Argonaute 2 cross-linking and immunoprecipitation data. We show that a substantial fraction of human miRNA target sites are noncanonical and that predicted target-site affinity correlates well with the extent of target destabilization. Our model provides a rigorous biophysical approach to miRNA target identification beyond ad hoc miRNA seed-based methods.
摘要
我们提出了一个 miRNA 与靶标相互作用的生物物理模型，并从 Argonaute 2 交联和免疫沉淀数据中推断其参数。我们表明，相当一部分人类 miRNA 靶标位点是非规范的，并且预测的靶标结合亲和力与靶标失稳的程度很好地相关。我们的模型为 miRNA 靶标识别提供了一种严格的生物物理方法，超越了基于 miRNA 种子的特定方法。
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