临床标本中 Abbott RealTime HBV 病毒载量检测试剂盒所针对的区域的序列保守性。
Sequence conservation of the region targeted by the Abbott RealTime HBV viral load assay in clinical specimens.
机构信息
Abbott Molecular, Des Plaines, Illinois, USA.
出版信息
J Clin Microbiol. 2013 Apr;51(4):1260-2. doi: 10.1128/JCM.03003-12. Epub 2013 Jan 23.
Abstract
The Abbott RealTime HBV assay targets the N-terminal region of the S gene. Here we analyzed the sequence variability of the assay target region from >2,100 clinical specimens. Thermodynamic modeling of the percentage of bound primer/probe at the assay annealing temperature was performed to assess the potential effect of sequence variability.
摘要
Abbott RealTime HBV 检测法针对 S 基因的 N 端区域。在这里，我们分析了 2100 多个临床标本中检测靶区域的序列变异性。通过对检测退火温度下结合引物/探针的百分比进行热力学建模，评估了序列变异性的潜在影响。
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