用于研究对人淋巴细胞生物学效应的全血太赫兹辐照
THz Exposure of Whole Blood for the Study of Biological Effects on Human Lymphocytes.
作者信息
Scarfì M R, Romanò M, Di Pietro R, Zeni O, Doria A, Gallerano G P, Giovenale E, Messina G, Lai A, Campurra G, Coniglio D, D'Arienzo M
机构信息
CNR-IREA, Via Diocleziano 328, 80124 Napoli (I).
出版信息
J Biol Phys. 2003 Jun;29(2-3):171-6. doi: 10.1023/A:1024440708943.
Abstract
The aim of the present study is toinvestigate the genotoxic effect of THzradiation in human peripheral bloodlymphocytes following 20 minutes exposureto 1 mW average power Free Electron Laserradiation in the frequency range 120-140GHz. For this purpose 9 healthy donors wereemployed and cytokinesis block techniquewas applied to study micronucleusfrequency and cell proliferation. Theresults obtained indicate that all theelectromagnetic conditions adopted so far do not alter the investigated parameters,suggesting absence of direct chromosomaldamage and alteration of cell cyclekinetics (two tailed paired Student's test:p> 0.05 in all cases).
摘要
本研究的目的是调查在120 - 140GHz频率范围内，平均功率为1mW的自由电子激光辐射照射20分钟后，太赫兹辐射对人外周血淋巴细胞的遗传毒性作用。为此，招募了9名健康供者，并应用胞质分裂阻滞技术来研究微核频率和细胞增殖。所获得的结果表明，迄今为止采用的所有电磁条件均未改变所研究的参数，这表明不存在直接的染色体损伤和细胞周期动力学改变（双尾配对学生检验：所有情况下p>0.05）。
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本文引用的文献
1
Absorption and Diffusion Measurements of Biological Samples using a THz Free Electron Laser.使用太赫兹自由电子激光对生物样品进行吸收和扩散测量。
J Biol Phys. 2003 Jun;29(2-3):159-70. doi: 10.1023/A:1024488624873.
2
Genotoxicity of radiofrequency signals. I. Investigation of DNA damage and micronuclei induction in cultured human blood cells.射频信号的遗传毒性。I. 培养的人血细胞中DNA损伤和微核诱导的研究。
Bioelectromagnetics. 2002 Feb;23(2):113-26. doi: 10.1002/bem.104.
3
Genotoxic, carcinogenic and teratogenic effects of electromagnetic fields. Introduction and overview.
Mutat Res. 1997 Dec;387(3):165-71. doi: 10.1016/s1383-5742(97)00036-7.
4
The cytokinesis-block micronucleus technique: a detailed description of the method and its application to genotoxicity studies in human populations.
Mutat Res. 1993 Jan;285(1):35-44. doi: 10.1016/0027-5107(93)90049-l.
5
Induction of micronuclei by five pyrethroid insecticides in whole-blood and isolated human lymphocyte cultures.
Mutat Res. 1995 Jan;341(3):169-84. doi: 10.1016/0165-1218(95)90007-1.
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