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同步辐射红外显微光谱学的生物学与生物医学应用

Biological and biomedical applications of synchrotron infrared microspectroscopy.

作者信息

Dumas P, Miller L

机构信息

LURE - Bat 209D -, Centre Universitaire Paris-Sud, F-91898 Orsay cédex, France.

出版信息

J Biol Phys. 2003 Jun;29(2-3):201-18. doi: 10.1023/A:1024448910761.

Abstract

The high brightness of synchrotron light,which is about three orders of magnitudegreater than a thermal source, has beenexploited in biological and biomedicalapplications of infrared microspectroscopy. The potential of this analytical tool isdocumented in this article in the study ofhuman tissue (hair and skin) and individualcells: biochemical and bio-structuralchanges based on corresponding functionalgroups have been identified and imaged withunprecedented spatial resolution. Thistechnique also provides a new tool foranalysis of biochemical kinetics of samplesduring disease and treatment. In the future, the combination ofinfrared microspectroscopy with othersynchrotron-based microscopic techniques,such as X-ray microscopy, at the samesample location is discussed.

摘要

同步加速器光的高亮度比热光源大约高三个数量级,已在红外显微光谱的生物和生物医学应用中得到利用。本文记录了这种分析工具在人体组织(头发和皮肤)和单个细胞研究中的潜力:基于相应官能团的生化和生物结构变化已被识别并以前所未有的空间分辨率成像。该技术还为疾病和治疗过程中样品的生化动力学分析提供了一种新工具。未来,还讨论了在同一样品位置将红外显微光谱与其他基于同步加速器的显微技术(如X射线显微镜)相结合的问题。

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