Department of Veterinary Medical Sciences, Alma Mater University of Bologna, Italy.
Res Vet Sci. 2013 Jun;94(3):379-87. doi: 10.1016/j.rvsc.2012.11.020. Epub 2013 Jan 22.
Aim of the study was to verify the clinical and morphological effects of intra-articular stanozolol or placebo treatment, lasting 3 and 9 months, in sheep in which a femoro-tibial osteo-arthritis (OA) were surgically induced (medial bilateral meniscectomy). Twenty healthy sheep divided into four groups and two control animals group, after surgical medial bilateral meniscectomy, were weekly injected in femoral-tibial joint (FTJ) with stanozolol or placebo. Lameness evaluation was performed and synovial fluid was collected from all sheep at each treatment time. Necropsies were performed after 3 or 9 month as described in experimental design. Gross pathologies were described and specimen tissues collected from femoro-tibial articular joints were processed for routine histological examination. The gross anatomy of the FTJ was well-preserved in stanozolol-treated sheep; this also applied to the histological features of articular cartilage. Joint aseptic inflammation and fibrosis were observed in placebo-treated sheep, associated with a different degree of severity of condylar and tibial plate cartilage degeneration. Stanozolol intra-articular treatment reduces osteophytes formation and subchondral bone reaction and promotes articular cartilage regeneration.
本研究旨在验证关节内司坦唑醇或安慰剂治疗，持续 3 个月和 9 个月，对手术诱导（内侧双侧半月板切除术）的羊股胫关节（OA）的临床和形态学影响。20 只健康绵羊分为四组和两组对照动物组，在手术内侧双侧半月板切除术后，每周在股胫关节（FTJ）注射司坦唑醇或安慰剂。在每次治疗时对跛行进行评估，并从所有绵羊中收集滑液。根据实验设计，在 3 或 9 个月后进行尸检。描述大体病理学，并从股胫关节收集关节标本组织进行常规组织学检查。在司坦唑醇治疗的绵羊中，FTJ 的大体解剖结构保存良好; 这也适用于关节软骨的组织学特征。在安慰剂治疗的绵羊中观察到关节无菌性炎症和纤维化，伴有不同程度的髁和胫骨板软骨变性。关节内司坦唑醇治疗可减少骨赘形成和软骨下骨反应，并促进关节软骨再生。
