使用牙菌斑微生态生物膜模型研究唾液薄膜对新型抗菌牙科粘合剂抗菌活性的影响。
Effect of salivary pellicle on antibacterial activity of novel antibacterial dental adhesives using a dental plaque microcosm biofilm model.
作者信息
Li Fang, Weir Michael D, Fouad Ashraf F, Xu Hockin H K
机构信息
Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, China; Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA.
Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA.
出版信息
Dent Mater. 2014 Feb;30(2):182-91. doi: 10.1016/j.dental.2013.11.004. Epub 2013 Dec 11.
OBJECTIVES
Antibacterial primer and adhesive are promising to inhibit biofilms and caries. Since restorations in vivo are exposed to saliva, one concern is the attenuation of antibacterial activity due to salivary pellicles. The objective of this study was to investigate the effects of salivary pellicles on bonding agents containing a new monomer dimethylaminododecyl methacrylate (DMADDM) or nanoparticles of silver (NAg) against biofilms for the first time.
METHODS
DMADDM and NAg were synthesized and incorporated into Scotchbond Multi-Purpose adhesive and primer. Specimens were either coated or not coated with salivary pellicles. A microcosm biofilm model was used with mixed saliva from ten donors. Two types of culture medium were used: an artificial saliva medium (McBain), and Brain Heart Infusion (BHI) medium without salivary proteins. Metabolic activity, colony-forming units (CFU), and lactic acid production of plaque microcosm biofilms were measured (n=6).
RESULTS
Bonding agents containing DMADDM and NAg greatly inhibited biofilm activities, even with salivary pellicles. When using BHI, the pre-coating of salivary pellicles on resin surfaces significantly decreased the antibacterial effect (p<0.05). When using artificial saliva medium, pre-coating of salivary pellicles on resin did not decrease the antibacterial effect. These results suggest that artificial saliva yielded medium-derived pellicles on resin surfaces, which provided attenuating effects on biofilms similar to salivary pellicles. Compared with the commercial control, the DMADDM-containing bonding agent reduced biofilm CFU by about two orders of magnitude.
SIGNIFICANCE
Novel DMADDM- and NAg-containing bonding agents substantially reduced biofilm growth even with salivary pellicle coating on surfaces, indicating a promising usage in saliva-rich environment. DMADDM and NAg may be useful in a wide range of primers, adhesives and other restoratives to achieve antibacterial and anti-caries capabilities.
目的
抗菌底漆和粘合剂有望抑制生物膜和龋齿。由于体内修复体暴露于唾液中，一个担忧是唾液薄膜会导致抗菌活性减弱。本研究的目的是首次研究唾液薄膜对含有新型单体甲基丙烯酸二甲氨基十二烷基酯（DMADDM）或纳米银（NAg）的粘合剂抗生物膜的影响。
方法
合成DMADDM和NAg并将其加入到Scotchbond多功能粘合剂和底漆中。样本涂有或未涂有唾液薄膜。使用来自十位捐赠者的混合唾液建立微观生物膜模型。使用两种培养基：人工唾液培养基（McBain）和不含唾液蛋白的脑心浸液（BHI）培养基。测量菌斑微观生物膜的代谢活性、菌落形成单位（CFU）和乳酸产量（n = 6）。
结果
含有DMADDM和NAg的粘合剂即使在有唾液薄膜的情况下也能极大地抑制生物膜活性。使用BHI时，树脂表面预先涂覆唾液薄膜会显著降低抗菌效果（p<0.05）。使用人工唾液培养基时，树脂表面预先涂覆唾液薄膜不会降低抗菌效果。这些结果表明，人工唾液在树脂表面产生了与培养基来源的薄膜，其对生物膜的减弱作用与唾液薄膜相似。与商业对照相比，含DMADDM的粘合剂使生物膜CFU减少了约两个数量级。
意义
新型含DMADDM和NAg的粘合剂即使在表面涂有唾液薄膜的情况下也能显著减少生物膜生长，表明在唾液丰富的环境中有良好的应用前景。DMADDM和NAg可能在广泛的底漆、粘合剂和其他修复材料中发挥作用，以实现抗菌和防龋能力。
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