CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, 4485-661 Vairão, Portugal.
Infect Genet Evol. 2013 Jun;16:38-53. doi: 10.1016/j.meegid.2013.01.009. Epub 2013 Jan 26.
Multi-locus sequence typing (MLST) is a high-resolution genetic typing approach to identify species and strains of pathogens impacting human health, agriculture (animals and plants), and biosafety. In this review, we outline the general concepts behind MLST, molecular approaches for obtaining MLST data, analytical approaches for MLST data, and the contributions MLST studies have made in a wide variety of areas. We then look at the future of MLST and their relative strengths and weaknesses with respect to whole genome sequence typing approaches that are moving into the research arena at an ever-increasing pace. Throughout the paper, we provide exemplar references of these various aspects of MLST. The literature is simply too vast to make this review comprehensive, nevertheless, we have attempted to include enough references in a variety of key areas to introduce the reader to the broad applications and complications of MLST data.
多位点序列分型（MLST）是一种高分辨率的遗传分型方法，用于鉴定影响人类健康、农业（动植物）和生物安全的病原体的物种和菌株。在这篇综述中，我们概述了 MLST 的一般概念、获得 MLST 数据的分子方法、MLST 数据的分析方法以及 MLST 研究在广泛领域中的贡献。然后，我们探讨了 MLST 的未来及其相对于全基因组序列分型方法的相对优势和劣势，后者正以越来越快的速度进入研究领域。在整篇论文中，我们提供了这些各种 MLST 方面的范例参考。由于文献太多，无法使本综述全面，但是，我们已经尝试在多个关键领域中包含足够的参考文献，以使读者了解 MLST 数据的广泛应用和复杂性。
