College of Life Science, Henan Normal University, Xinxiang 453007, China.
Chin J Cancer Res. 2011 Dec;23(4):301-5. doi: 10.1007/s11670-011-0301-2.
Based on liver cancer model built in SD rats, the contents of trace elements (Cu, Fe, Zn, Ca and Mg), AFP, CEA, SF, TH and IGF-II in serum were measured at different stages to explore the molecular changes during the rat liver cancer development.
The SD rat liver cancer model was built by using diethylnitrosamine (DENA) as the mutagen. During 16 weeks of DENA gavage, blood samples were taken in the 14th, 28th, 56th, 77th, 105th and 112th days respectively after the first day of gavage with DENA, then the contents of five trace elements (Cu, Fe, Zn, Ca and Mg), T3, T4, IGF-II, AFP, CEA and SF in serum were determined.
During the development of the rat liver cancer, in the test group, the Cu content significantly increased in serum, while the contents of Fe, Zn and Ca significantly decreased. The content of Mg showed no significant change. AFP and CEA of the test group showed same expression level with the control group; while the content of SF was lower than that of the control group when cancerization appeared. T3 and T4 increased at the first stage and then went down, and the content of IGF-II was always high.
Cu, Fe, Zn, Ca, T3, T4, SF and IGF-II are closely related to the development of liver cancer. The changes of their contents in the development of cancer could enlighten the researches on cancer pathogenesis and prevention.
基于建立的 SD 大鼠肝癌模型，检测不同阶段血清中微量元素（Cu、Fe、Zn、Ca、Mg）、甲胎蛋白（AFP）、癌胚抗原（CEA）、铁蛋白（SF）、三碘甲状腺原氨酸（TH）和胰岛素样生长因子-II（IGF-II）的含量，探讨肝癌发生发展过程中的分子变化。
采用二乙基亚硝胺（DENA）作为诱变剂构建 SD 大鼠肝癌模型。DENA 灌胃 16 周期间，在 DENA 灌胃第 1 天的第 14、28、56、77、105 和 112 天分别取血，检测血清中 5 种微量元素（Cu、Fe、Zn、Ca、Mg）、T3、T4、IGF-II、AFP、CEA 和 SF 的含量。
在大鼠肝癌发生发展过程中，实验组血清 Cu 含量明显升高，而 Fe、Zn 和 Ca 含量明显降低，Mg 含量无明显变化。实验组 AFP 和 CEA 与对照组表达水平相同；而当癌变出现时，SF 的含量低于对照组。T3 和 T4 在第一阶段增加然后下降，IGF-II 的含量一直较高。
Cu、Fe、Zn、Ca、T3、T4、SF 和 IGF-II 与肝癌的发生发展密切相关。这些物质在癌症发展过程中的含量变化可以为癌症发病机制和预防的研究提供启示。
