Li Wei, Ding Yan, Sun Ya Nan, Yan Xi Tao, Yang Seo Young, Choi Chun Whan, Cha Ji Yun, Lee Young Mi, Kim Young Ho
College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea
Chem Pharm Bull (Tokyo). 2013;61(4):471-6. doi: 10.1248/cpb.c12-01034. Epub 2013 Jan 25.
In the present study, a new oleanane-type triterpenoid saponin, pulsatilloside F (1), along with 21 known compounds (2-22), were isolated from the root of Pulsatilla koreana. Their chemical structures were elucidated by mass, (1)H-, (13)C-NMR, correlation spectroscopy (COSY), heteronuclear multiple quantum coherence (HMQC) and heteronuclear multiple bond connectivity (HMBC) spectroscopy. Anti-inflammatory effects of the compounds were evaluated in terms of inhibitory of tumor necrosis factor α (TNF-α) secretion in the lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophage cell line. Compounds 19 and 20 exhibited particularly inhibitory effects with respective IC50 values of 0.32 and 0.65 µm. Compounds 1-4, 7 and 10-13 exhibited inhibitory effects with inhibition rates up to 41.55-73.76% at a concentration of 5 µm, respectively.
在本研究中，从朝鲜白头翁的根部分离出一种新的齐墩果烷型三萜皂苷——白头翁皂苷F（1），以及21种已知化合物（2 - 22）。通过质谱、氢谱、碳谱、相关谱（COSY）、异核多量子相干谱（HMQC）和异核多键相关谱（HMBC）对它们的化学结构进行了阐明。根据对脂多糖（LPS）刺激的小鼠RAW264.7巨噬细胞系中肿瘤坏死因子α（TNF-α）分泌的抑制作用，评估了这些化合物的抗炎效果。化合物19和20表现出特别的抑制作用，其IC50值分别为0.32和0.65µm。化合物1 - 4、7以及10 - 13在浓度为5µm时表现出抑制作用，抑制率分别高达41.55 - 73.76%。
