College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea.
Molecules. 2013 May 8;18(5):5306-16. doi: 10.3390/molecules18055306.
Pulsatilla koreana, a species endemic to Korea, is an important herb used in traditional medicine to treat amoebic dysentery and malaria. In the present study, 23 oleanane-type triterpenoid saponins 1-23 and eight lupane-type triterpenoid saponins 24-31 were isolated from the roots of P. koreana. Their structures were elucidated on the basis of spectroscopic data. The methanol extract and isolated compounds were next assessed for nematicidal activity against the root-knot nematode (Meloidogyne incognita). The methanol extract showed strong nematicidal activity after 48 h, with a LC₅₀ value of 92.8 μg/mL. Compounds 2, 5, 9, 20, and 21 showed significant effects, with LC₅₀ values ranging from 70.1 to 94.7 μg/mL after 48 h. These results suggest that triterpenoid saponins from P. koreana should be explored as potential natural nematicides for developing new agents to control root-knot nematode disease.
朝鲜白头翁是一种原产于朝鲜的物种，是一种重要的草药，用于治疗阿米巴痢疾和疟疾。在本研究中，从朝鲜白头翁的根部分离得到了 23 种齐墩果烷型三萜皂苷 1-23 和 8 种羽扇豆烷型三萜皂苷 24-31。基于光谱数据阐明了它们的结构。接下来评估了甲醇提取物和分离得到的化合物对根结线虫（Meloidogyne incognita）的杀线虫活性。甲醇提取物在 48 小时后表现出很强的杀线虫活性，LC₅₀值为 92.8 μg/mL。化合物 2、5、9、20 和 21 在 48 小时后表现出显著的效果，LC₅₀值在 70.1 到 94.7 μg/mL 之间。这些结果表明，朝鲜白头翁中的三萜皂苷类化合物有望作为潜在的天然杀线虫剂，用于开发控制根结线虫病的新型药剂。
