Washington University in St. Louis, Department of Biomedical Engineering, One Brookings Drive, St. Louis, MO 63130, USA.
J Biomed Opt. 2013 Feb;18(2):26003. doi: 10.1117/1.JBO.18.2.026003.
A novel photoacoustic thermometric method is presented for simultaneously imaging cells and sensing their temperature. With three-seconds-per-frame imaging speed, a temperature resolution of 0.2°C was achieved in a photo-thermal cell heating experiment. Compared to other approaches, the photoacoustic thermometric method has the advantage of not requiring custom-developed temperature-sensitive biosensors. This feature should facilitate the conversion of single-cell thermometry into a routine lab tool and make it accessible to a much broader biological research community.
本文提出了一种新型的光声测温方法，可用于同时对细胞进行成像和感测其温度。在光热细胞加热实验中，该方法实现了 0.2°C 的温度分辨率，帧率为 3 帧/秒。与其他方法相比，光声测温法的优势在于不需要定制开发的温度敏感生物传感器。这一特点应该有助于将单细胞测温技术转化为常规实验室工具，并使其更广泛地应用于生物学研究领域。
