Proteomics Unit, Department of Biomedicine, University of Bergen, Bergen, Norway.
Amino Acids. 2013 Apr;44(4):1129-37. doi: 10.1007/s00726-012-1455-z. Epub 2013 Feb 2.
With the growing interest in the field of proteomics, the amount of publicly available proteome resources has also increased dramatically. This means that there are many useful resources available for almost all aspects of a proteomics experiment. However, it remains vital to use the right resource, for the right purpose, at the right time. This review is therefore meant to aid the reader in obtaining an overview of the available resources and their application, thus providing the necessary background to choose the appropriate resources for the experiment at hand. Many of the resources are also taking advantage of so-called crowdsourcing to maximize the potential of the resource. What this means and how this can improve future experiments will also be discussed. The text roughly follows the steps involved in a proteomics experiment, starting with the planning of the experiment, via the processing of the data and the analysis of the results, to the community-wide sharing of the produced data.
随着人们对蛋白质组学领域的兴趣日益浓厚，公开可用的蛋白质组资源也大幅增加。这意味着几乎在蛋白质组学实验的各个方面都有许多有用的资源可用。然而，在正确的时间使用正确的资源仍然至关重要。因此，本文旨在帮助读者了解可用资源及其应用，从而为选择手头实验的适当资源提供必要的背景。许多资源还利用所谓的众包来最大限度地发挥资源的潜力。本文还将讨论这意味着什么以及这如何改进未来的实验。本文大致按照蛋白质组学实验所涉及的步骤进行，从实验规划开始，到数据处理和结果分析，再到产生的数据在整个社区的共享。
