Robier Christoph, Quehenberger Franz, Neubauer Manfred, Stettin Mariana, Rainer Franz
Central Laboratory, Department of Internal Medicine, Hospital Barmherzige Brueder Graz-Eggenberg, Bergstrasse 27, 8020, Graz, Austria,
Rheumatol Int. 2014 Jun;34(6):773-6. doi: 10.1007/s00296-013-2689-0. Epub 2013 Feb 7.
In synovial fluids (SF) with low leukocyte or/and crystal counts, important features may be missed, if exclusively smears are examined by polarized microscopy. That may be overcome by cytocentrifuges, which use low-speed centrifugal force to concentrate cells onto a glass slide and thus enhance the number of cells per high power field (HPF). We compared the calcium pyrophosphate (CPP) crystal counts in cytospin preparations with those in common smears of SF. The number of CPP crystals was counted in 50 SF samples by polarized microscopy, and statistical comparisons of the mean values of the cytospin and smear preparations were performed using the Wilcoxon test. The reproducibility within the slides of the cytocentrifuge and smear samples was determined by Spearman's rank correlation. The crystal counts were significantly higher in the cytospin than in the smear preparations (median 96/10 HPF vs. 2.5/10 HPF, p < 0.0001). The correlation in the crystal count between the slides 1 and 2 was significantly higher within the cytocentrifuge than in the smear group (0.97 vs. 0.73, p = 0.0004). CPP-negative cytospin preparations in initially smear-positive slides were not observed. We confirmed that the cytospin technique significantly enhances the number of examinable crystals per HPF, compared to common smears.
在白细胞计数低和/或晶体计数低的滑液（SF）中，如果仅通过偏振显微镜检查涂片，可能会遗漏重要特征。这可以通过细胞离心仪来克服，细胞离心仪利用低速离心力将细胞浓缩到载玻片上，从而增加每个高倍视野（HPF）中的细胞数量。我们比较了细胞离心涂片制剂和普通滑液涂片制剂中的焦磷酸钙（CPP）晶体计数。通过偏振显微镜对50份滑液样本中的CPP晶体数量进行计数，并使用Wilcoxon检验对细胞离心涂片和普通涂片制剂的平均值进行统计学比较。通过Spearman秩相关确定细胞离心仪涂片样本和普通涂片样本在载玻片内的重复性。细胞离心涂片制剂中的晶体计数显著高于普通涂片制剂（中位数分别为96/10 HPF和2.5/10 HPF，p < 0.0001）。细胞离心仪组中载玻片1和载玻片2之间的晶体计数相关性显著高于普通涂片组（0.97对0.73，p = 0.0004）。在最初涂片为阳性的载玻片中未观察到细胞离心涂片制剂为CPP阴性的情况。我们证实，与普通涂片相比，细胞离心涂片技术显著增加了每个HPF中可检查的晶体数量。
