PNA-FISH 酵母交通灯快速鉴定血培养阳性标本中酵母及临床影响评估
Evaluation of PNA-FISH yeast traffic light for rapid identification of yeast directly from positive blood cultures and assessment of clinical impact.
机构信息
Department of Microbiology, Royal Free Hospital, London, United Kingdom.
出版信息
J Clin Microbiol. 2013 Apr;51(4):1301-2. doi: 10.1128/JCM.00028-13. Epub 2013 Feb 6.
Abstract
The PNA-FISH Yeast Traffic Light assay was performed on 54 clinical isolates of yeasts inoculated into blood culture bottles. The assay showed high sensitivity (Candida albicans/C. parapsilosis, 100%; C. glabrata/C. krusei, 92.3%; C. tropicalis, 100%) and specificity (C. albicans/C. parapsilosis, 100%; C. glabrata/C. krusei, 94.8%; C. tropicalis, 100%). Case note review estimated a change in therapy in 29% of cases had the PNA-FISH result been available to the clinician.
摘要
对 54 株临床分离酵母接种到血培养瓶中进行 PNA-FISH 酵母交通灯检测。该检测方法具有较高的灵敏度（白念珠菌/近平滑念珠菌，100%；光滑念珠菌/克柔念珠菌，92.3%；热带念珠菌，100%）和特异性（白念珠菌/近平滑念珠菌，100%；光滑念珠菌/克柔念珠菌，94.8%；热带念珠菌，100%）。病例记录回顾估计，如果临床医生可以获得 PNA-FISH 结果，那么 29%的病例可能会改变治疗方案。
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