Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, 981-8558, Japan.
J Nat Med. 2013 Oct;67(4):867-75. doi: 10.1007/s11418-013-0748-1. Epub 2013 Feb 9.
Activity-guided isolation of the n-butanol fraction of Chamaerhodos erecta and water soluble fraction of C. altaica resulted in the isolation of 39 compounds, including new compounds identified as 4,5-dihydroxybenzaldehyde-3-O-β-D-glucopyranoside (1) from C. erecta and quercetin-3-O-β-D-glucuronopyranosyl-4'-O-β-D-glucopyranoside (2) from C. altaica. A total of 37 other compounds were identified based on physico-chemical properties and spectroscopic data. Antioxidative activity was evaluated using a DPPH radical-scavenging method, hyaluronidase inhibitory activity, and advanced glycation end products production inhibitory activity of isolated compounds. Some flavonols (4, 6, 9-11, 14, 15), catechins (18, 19), an amino acid (20), a lignan glucoside (23), and tannins (29-39) exhibited potential a free radical scavenging activity while the new compound (1) showed weak activity. A catechin (18) and some of the tannins (32, 33, 35, 36, 38) had moderate hyaluronidase inhibitory activity. Some of flavonoids and tannins prevented advanced glycation end products production, and the IC₅₀ of compounds 3, 9, 14-16, 33, 34, 36, 38, and 39 were determined.
从黄花木（Chamaerhodos erecta）的正丁醇部位和金老梅（C. altaica）的水溶性部位经活性导向分离得到 39 种化合物，包括从黄花木中分离得到的新化合物 4,5-二羟基苯甲醛-3-O-β-D-吡喃葡萄糖苷（1）和从金老梅中分离得到的槲皮素-3-O-β-D-吡喃葡萄糖醛酸基-4'-O-β-D-吡喃葡萄糖苷（2）。根据理化性质和光谱数据共鉴定出 37 种其他化合物。采用 DPPH 自由基清除法、透明质酸酶抑制活性和晚期糖基化终产物生成抑制活性评价分离得到的化合物的抗氧化活性。一些黄酮醇（4、6、9-11、14、15）、儿茶素（18、19）、一种氨基酸（20）、一种木脂素葡萄糖苷（23）和单宁（29-39）表现出潜在的自由基清除活性，而新化合物（1）表现出较弱的活性。一种儿茶素（18）和一些单宁（32、33、35、36、38）具有中等的透明质酸酶抑制活性。一些类黄酮和单宁可预防晚期糖基化终产物的产生，测定了化合物 3、9、14-16、33、34、36、38 和 39 的 IC₅₀。
