Department of Chemistry, National Institute of Technology, Durgapur, WB, India.
J Fluoresc. 2013 May;23(3):495-501. doi: 10.1007/s10895-013-1169-y. Epub 2013 Feb 10.
A rhodamine B-based fluorescence probe (1) for the sensitive and selective detection of Cu(2+) ion has been designed and synthesized using pyridine moiety. The optical properties of this compound have been investigated in acetonitrile-water binary solution (7:3 v/v). Compound 1 is found to be an excellent sensor for a biologically/physiologically very important transition metal ion (Cu(2+)) using only the two very different modes of measurements (absorption and emission); one case displayed intensity enhancement whereas in other case showed intensity depletion (quenching). A mechanistic investigation has been performed to explore the static nature of quenching process. The sensor has been found to be very effective in sensing Cu(2+) ion inside living cells also.
一种基于罗丹明 B 的荧光探针（1）已被设计和合成，用于灵敏和选择性检测 Cu(2+)离子，使用了吡啶部分。该化合物的光学性质已在乙腈-水二元溶液（7:3 v/v）中进行了研究。化合物 1 被发现是一种非常重要的过渡金属离子（Cu(2+)）的生物/生理传感器，仅使用两种非常不同的测量模式（吸收和发射）；一种情况显示出强度增强，而另一种情况则显示出强度耗尽（猝灭）。进行了机制研究以探索猝灭过程的静态性质。该传感器也被发现非常有效地用于检测活细胞内的 Cu(2+)离子。
