Department of Clinical Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY 14853, USA.
J Vet Intern Med. 2013 Mar-Apr;27(2):371-6. doi: 10.1111/jvim.12046. Epub 2013 Feb 11.
The endurance sled dog is the ultimate endurance athlete in which to examine the exercise-associated acute phase and myokine responses that might be related to changes in muscle metabolism and damage. An inciting cause for increased C-reactive protein has yet to be elucidated, which might involve interleukin-6 and other myokines.
To examine concentrations of interleukin-6 (IL-6), interleukin-15 (IL-15), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α), and markers of the inflammatory response of exercise; monocyte chemoattractant protein-1(MCP-1) and C-reactive protein (CRP) before, during, and after an endurance racing event.
26 sled dogs completing a 1650-km race.
In a prospective study, cephalic venipuncture was performed before racing, at the midpoint, and after racing. Body weight and serum CRP, MCP-1, IL-15, IL-8, IL-6, and TNF-α concentrations were evaluated using enzyme-linked immunoabsorbance-based assays or a luminex multiplex assay.
There were no significant differences in concentrations of IL-6, IL-15, IL-8, or TNF-α at the 3 time points, whereas there were significant increases in MCP-1 (median and range-start: 86 pg/mL [30-1845]; midpoint: 179 pg/mL [53-730]; finish: 180 pg/mL [21-1294]; P < .01) and CRP (median and range-start: 18 μg/mL [11-58]; midpoint: 76 μg/mL [12-198]; finish: 60 μg/mL [12-170]; P < .01) at the midpoint and race finish. There was a significant linear relationship between MCP-1 and IL-6 (R = 0.68; P < .01).
The inflammatory response to exercise increases as measured by MCP-1 during and after endurance exercise in sled dogs. IL-6 appears to be associated with MCP-1; however, the reasons for increases in the acute phase response (CRP) cannot be attributed to IL-6 or other myokines. IL-6 and MCP-1 concentrations might be useful in future investigations of exertional rhabdomyolysis.
耐力雪橇犬是终极耐力运动员，可用于检查与肌肉代谢和损伤变化相关的运动相关急性期和肌肉因子反应。尚未阐明 C 反应蛋白升高的激发原因，这可能涉及白细胞介素-6 和其他肌肉因子。
检查白细胞介素-6 (IL-6)、白细胞介素-15 (IL-15)、白细胞介素-8 (IL-8) 和肿瘤坏死因子-α (TNF-α) 以及运动引起的炎症反应标志物；在耐力赛前后的单核细胞趋化蛋白-1 (MCP-1) 和 C 反应蛋白 (CRP) 的浓度。
26 只完成 1650 公里比赛的雪橇犬。
在一项前瞻性研究中，在比赛前、中途和比赛后进行头部静脉穿刺。使用酶联免疫吸附测定法或 Luminex 多重测定法评估体重和血清 CRP、MCP-1、IL-15、IL-8、IL-6 和 TNF-α 浓度。
在 3 个时间点，IL-6、IL-15、IL-8 或 TNF-α 的浓度没有显着差异，而 MCP-1（中位数和范围开始：86 pg/mL [30-1845]；中间：179 pg/mL [53-730]；完成：180 pg/mL [21-1294]；P <.01）和 CRP（中位数和范围开始：18 μg/mL [11-58]；中间：76 μg/mL [12-198]；完成：60 μg/mL [12-170]；P <.01）在中途和比赛结束时显着增加。MCP-1 和 IL-6 之间存在显着的线性关系（R = 0.68；P <.01）。
在雪橇犬的耐力运动中，通过 MCP-1 测量，运动引起的炎症反应增加。IL-6 似乎与 MCP-1 相关；然而，急性期反应（CRP）增加的原因不能归因于 IL-6 或其他肌肉因子。IL-6 和 MCP-1 浓度可能有助于未来对运动性横纹肌溶解症的研究。
