Department of Oral Microbiology and Institute of Oral Bioscience, Chonbuk National University, Jeonju, South Korea.
Arch Oral Biol. 2013 May;58(5):482-92. doi: 10.1016/j.archoralbio.2012.11.002. Epub 2013 Feb 8.
Fucoidan is a sulphated polysaccharide that is primarily extracted from brown seaweeds; it has been broadly studied in recent years due to its numerous biological properties, including anticoagulant, antithrombotic, antitumour and antiviral activities.
In this study, fucoidan was evaluated against oral bacteria, either alone or with antibiotics, via the broth dilution method and chequerboard and time-kill assay.
Minimum inhibitory concentration/minimum bactericidal concentration (MIC/MBC) values for the fucoidan against all the tested bacteria ranged between 0.125 and 0.50/0.25 and 1.00mgml(-1), for ampicillin 0.125 and 64/0.5 and 64μgml(-1) and for gentamicin 2 and 256/4 and 512μgml(-1), respectively. Furthermore, the MIC and MBC were reduced to one half-eighth as a result of the combination of the fucoidan with antibiotics. One to 3h of treatment with MIC50 of fucoidan with MIC50 of antibiotics resulted from an increase of the rate of killing in colony forming units (CFUs) ml(-1) to a greater degree than was observed with alone.
These results suggest that fucoidan is important in the antibacterial actions of the agents.
褐藻糖胶是一种主要从褐藻中提取的硫酸多糖，近年来由于其众多的生物特性，包括抗凝、抗血栓、抗肿瘤和抗病毒活性，受到了广泛的研究。
本研究通过肉汤稀释法和棋盘法及时间杀伤试验，单独或联合抗生素评估褐藻糖胶对口腔细菌的作用。
褐藻糖胶对所有测试细菌的最低抑菌浓度/最低杀菌浓度（MIC/MBC）值在 0.125 至 0.50/0.25 和 1.00mgml(-1)之间，氨苄西林为 0.125 和 64/0.5 和 64μgml(-1)，庆大霉素为 2 和 256/4 和 512μgml(-1)。此外，褐藻糖胶与抗生素联合使用后，MIC 和 MBC 降低至原来的一半至八分之一。用 MIC50 的褐藻糖胶和 MIC50 的抗生素处理 1 至 3 小时，导致菌落形成单位（CFU）ml(-1)的杀灭率增加程度大于单独使用时。
这些结果表明，褐藻糖胶在这些药物的抗菌作用中很重要。
