Sun Yat-Sen University, The First Affiliated Hospital, Department of Emergency, Guangzhou, People's Republic of China.
Clinics (Sao Paulo). 2013 Jan;68(1):75-80. doi: 10.6061/clinics/2013(01)oa12.
Recent studies have shown that circulating microRNAs might be useful, novel biomarkers for the diagnosis of acute myocardial infarction. The aims of this study were to evaluate the expression of cardiac-specific miRNAs (miR-1, -133a, -208b, and -499) in patients with acute myocardial infarction and to compare the diagnostic values of these miRNAs with that of cardiac troponin T.
Sixty-seven plasma samples obtained from patients with acute myocardial infarction and 32 plasma specimens collected from healthy volunteers were analyzed in this study. The levels of cardiac-specific miRNAs (miR-1, -133a, -208b, and -499) were measured by quantitative reverse transcription-polymerase chain reaction, and the concentrations of plasma cardiac troponin T were measured using electrochemiluminescence-based methods and an Elecsys 2010 Immunoassay Analyzer.
The levels of plasma miR-1, -133a, -208b, and -499 were significantly higher in acute myocardial infarction patients (all p<0.001) than in healthy volunteers. The expression of the cardiac-specific miRNAs in acute myocardial infarction patients decreased to close to the baseline levels at the time of hospital discharge (all p>0.05). There were no correlations between the levels of the four circulating miRNAs and the clinical characteristics of the study population (all p>0.05). Furthermore, receiver operating characteristic curve analyses showed that the four plasma miRNAs were not superior to cardiac troponin T for the diagnosis of acute myocardial infarction (all p>0.05).
Our results demonstrate that circulating miR-1, -133a, -208b, and -499 may be useful biomarkers in acute myocardial infarction patients but that these miRNAs are not superior to cardiac troponin T for the diagnosis of acute myocardial infarction.
最近的研究表明，循环 microRNAs 可能是急性心肌梗死诊断的有用的新型生物标志物。本研究旨在评估急性心肌梗死患者中心脏特异性 microRNAs（miR-1、-133a、-208b 和-499）的表达，并比较这些 microRNAs 与心肌肌钙蛋白 T 的诊断价值。
本研究分析了 67 例急性心肌梗死患者和 32 例健康志愿者的血浆样本。通过定量逆转录聚合酶链反应测定心脏特异性 microRNAs（miR-1、-133a、-208b 和-499）的水平，并使用基于电化学发光的方法和 Elecsys 2010 免疫分析测定仪测定血浆心肌肌钙蛋白 T 的浓度。
急性心肌梗死患者的血浆 miR-1、-133a、-208b 和-499 水平明显高于健康志愿者（均 p<0.001）。急性心肌梗死患者出院时，心脏特异性 microRNAs 的表达降至接近基线水平（均 p>0.05）。四种循环 microRNAs 的水平与研究人群的临床特征之间无相关性（均 p>0.05）。此外，受试者工作特征曲线分析表明，四种血浆 microRNAs 用于诊断急性心肌梗死并不优于心肌肌钙蛋白 T（均 p>0.05）。
我们的结果表明，循环 miR-1、-133a、-208b 和-499 可能是急性心肌梗死患者有用的生物标志物，但这些 microRNAs 用于诊断急性心肌梗死并不优于心肌肌钙蛋白 T。
