Muni I A, Leeling J L, Helms R J, Johnson N, Bare J J, Phillips B M
J Pharm Sci. 1978 Feb;67(2):289-91. doi: 10.1002/jps.2600670251.
An improvement in a previously described method for the determination of plasma salicylic acid and aspirin levels in humans is described. The procedure was simplified by employing only one plasma sample for both salicylates. More accurate estimation of salicylates, particularly aspirin, was achieved by using two different calibration curves. Salicylic acid was estimated by reaction with an aqueous solution of the Folin-Ciocalteu phenol reagent. Absorbance of the blue-colored complex, which formed on addition of sodium hydroxide, was measured at 670 nm. The influence of alkalinity in the formation of the colored complex is discussed. The average recovery of aspirin added to plasma was 94.61%; it was 214.72% by the previous method.
本文描述了一种对先前所述人体血浆水杨酸和阿司匹林水平测定方法的改进。该程序通过仅使用一份血浆样本测定两种水杨酸盐而得以简化。通过使用两条不同的校准曲线，实现了对水杨酸盐，尤其是阿司匹林更准确的估算。水杨酸通过与福林-西奥尔特苯酚试剂水溶液反应进行估算。在加入氢氧化钠后形成的蓝色络合物的吸光度在670nm处测量。讨论了碱性对有色络合物形成的影响。添加到血浆中的阿司匹林的平均回收率为94.61%；而先前方法的回收率为214.72%。
