在 opaque 红球菌 PD630 中建立用于脂质生产的纤维二糖利用体系。
Establishment of cellobiose utilization for lipid production in Rhodococcus opacus PD630.
作者信息
Hetzler Stephan, Steinbüchel Alexander
机构信息
Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Münster, Germany.
出版信息
Appl Environ Microbiol. 2013 May;79(9):3122-5. doi: 10.1128/AEM.03678-12. Epub 2013 Feb 22.
Abstract
Rhodococcus opacus PD630, which is known for its ability to accumulate large amounts of triacylglycerols (TAG), was metabolically engineered, and a cellobiose utilization pathway was introduced. Activities of β-glucosidases were determined, and recombinant strains accumulated fatty acids up to 39.5 ± 5.7% (wt/wt) of cell dry mass from cellobiose.
摘要
以能够积累大量三酰甘油（TAG）而闻名的迟钝红球菌PD630进行了代谢工程改造，并引入了纤维二糖利用途径。测定了β-葡萄糖苷酶的活性，重组菌株从纤维二糖中积累的脂肪酸含量高达细胞干重的39.5±5.7%（重量/重量）。
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