School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Mol Cell Biochem. 2013 Jun;378(1-2):73-81. doi: 10.1007/s11010-013-1595-9. Epub 2013 Feb 26.
Alpha-lipoic acid (LA) is considered to be a universal antioxidant. The intestine is very sensitive to a wide range of stressors for it exposed in a complex microenvironment that usually contained excess free radicals. The objective of this study was to evaluate the pre-protective effect of LA on intestinal epithelial cell injury induced by H2O2. In this research, IPEC-J2 cells were divided into three groups: the control group, the H2O2-treated group, and the LA pre-protective group. Cell viability, intracellular ROS, DNA damage integrity, and antioxidant enzyme activity of all groups were measured. Quantitative reverse transcription-PCR was used to determine the influence of oxidative stress and LA on the GH/IGF-1 axis in IPEC-J2 cells. The research selected 1 mM H2O2 and 10 μg/ml as modeling concentration. The data indicated that H2O2 can significantly increase the intracellular free radicals, leading to serious DNA damage and significantly reduce superoxide dismutase, glutathione peroxidase, catalase, and lipase activity. LA-protected IPEC-J2 cells against H2O2-induced injury by scavenging hydroxyl radical. In the current study, LA pre-protective group share a lower intracellular ROS and lower DNA damage compare to H2O2-treated group (P < 0.01); and also, a higher lipase activity were detected in LA pre-protective group compare to H2O2-treated group (P < 0.05). But the mechanism between oxidative stress, LA effect, and GH/IGF-1 axis is still unknown. The model used in the current study is also suitable for other feed additive screening.
α-硫辛酸（LA）被认为是一种通用的抗氧化剂。肠道对各种应激原非常敏感，因为它暴露在通常含有过量自由基的复杂微环境中。本研究旨在评估 LA 对 H2O2 诱导的肠上皮细胞损伤的预先保护作用。在这项研究中，将 IPEC-J2 细胞分为三组：对照组、H2O2 处理组和 LA 预先保护组。测量所有组的细胞活力、细胞内 ROS、DNA 损伤完整性和抗氧化酶活性。定量逆转录 PCR 用于确定氧化应激和 LA 对 IPEC-J2 细胞 GH/IGF-1 轴的影响。研究选择 1mM H2O2 和 10μg/ml 作为建模浓度。数据表明，H2O2 可以显著增加细胞内自由基，导致严重的 DNA 损伤，并显著降低超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶和脂肪酶的活性。LA 通过清除羟自由基保护 IPEC-J2 细胞免受 H2O2 诱导的损伤。在本研究中，LA 预先保护组的细胞内 ROS 和 DNA 损伤明显低于 H2O2 处理组（P<0.01）；并且，LA 预先保护组的脂肪酶活性明显高于 H2O2 处理组（P<0.05）。但是，氧化应激、LA 作用和 GH/IGF-1 轴之间的机制尚不清楚。本研究中使用的模型也适用于其他饲料添加剂的筛选。
