Department of Chemistry, Center for Diagnostics and Therapeutics, and Center for Biotechnology and Drug Design Georgia State University, P.O. Box 4098, Atlanta, GA 30302-4098.
Chemistry. 2013 Mar 18;19(12):4036-4042. doi: 10.1002/chem.201201677. Epub 2013 Feb 28.
Post-synthesis modification of DNA is an important way of functionalizing DNA molecules. Herein, we describe a method that first enzymatically incorporates a cyanobenzothiazole (CBT)-modified thymidine. The side-chain handle CBT can undergo a rapid and site-specific cyclization reaction with 1,2-aminothiols to afford DNA functionalization in aqueous solution. Another key advantage of this method is the formation of a single stereo/regioisomer in the process, which allows for precise control of DNA modification to yield a single component for aptamer selection work and other applications.
DNA 的合成后修饰是功能化 DNA 分子的一种重要方式。在此，我们描述了一种方法，该方法首先通过酶促反应将氰基苯并噻唑（CBT）修饰的胸苷掺入 DNA 中。侧链手柄 CBT 可与 1,2-氨基硫醇快速且特异性地环化反应，从而在水溶液中对 DNA 进行功能化。该方法的另一个关键优势是在反应过程中形成单一的立体/区域异构体，从而可以精确控制 DNA 的修饰，以产生用于适体选择工作和其他应用的单一成分。
