Calli Caglar, Pinar Ercan, Oncel Semih, Alper Bagriyanik H, Umut Sakarya E
Department of Otolaryngology, Ataturk Training and Research Hospital, 116/17 Sok No: 7/1 Platin Konutlari Evka 3, Bornova, Izmir, 35160 Turkey.
Indian J Otolaryngol Head Neck Surg. 2012 Mar;64(1):46-50. doi: 10.1007/s12070-011-0160-7. Epub 2011 Feb 9.
The purpose of this study was to investigate the effectiveness of intratympanic dexamethasone injection as a therapeutic agent against cisplatin-induced ototoxicity. Animals were randomly divided into three groups. Group one received intraperitoneal cisplatin alone, group two, received intratympanic dexamethasone after cisplatin ototoxicity had been demonstrated. Group three, which is control group, received intratympanic dexamethasone.Then we made three measurements. First we measured the baseline distortion product otoacustic emission (DPOAEs) of all the guine pigs. Second we injected cisplatin intraperitoneal group one and two the same day. Third we measured DPOAEs after 72 h of group one and two. Moreover DPOAEs were measured at the end of the first and second week only in group two. Cochleae were harvested and processed for electron microscopy after then. Values of The DPOAEs amplitudes and signal-to-noise ratio (SNR) at 1-6 kHz frequencies for group 1 after the injections significantly decreased over those before injections (P < 0.05). In group 3, there were no significant differences in DPOAE amplitude and SNR values When they are compare before and after their intratympanic dexamethasone injections (P > 0.05). In group 2, the DPOAEs measurements were close to significance at the end of the second week (P = 0.056). Intratympanic dexamethasone injection did not cause any ototoxic effect. Although intratympanic dexamethasone did not reach the statistically significant results, the measurements were close to significance. Intratympanic dexamethasone might have a significant therapeutic effect after cisplatin ototoxicity with different dose and application regimens.
本研究的目的是探讨鼓室内注射地塞米松作为治疗顺铂所致耳毒性药物的有效性。动物被随机分为三组。第一组仅接受腹腔注射顺铂，第二组在顺铂耳毒性得到证实后接受鼓室内注射地塞米松。第三组作为对照组，接受鼓室内注射地塞米松。然后我们进行了三项测量。首先，我们测量了所有豚鼠的基线畸变产物耳声发射（DPOAE）。其次，我们在同一天对第一组和第二组腹腔注射顺铂。第三，我们在第一组和第二组注射72小时后测量DPOAE。此外，仅在第二组的第一周和第二周结束时测量DPOAE。之后收集耳蜗并进行电子显微镜处理。注射后第一组在1-6kHz频率下的DPOAE振幅和信噪比（SNR）值与注射前相比显著降低（P<0.05）。在第三组中，鼓室内注射地塞米松前后的DPOAE振幅和SNR值比较无显著差异（P>0.05）。在第二组中，第二周结束时DPOAE测量值接近显著性水平（P=0.056）。鼓室内注射地塞米松未引起任何耳毒性作用。虽然鼓室内注射地塞米松未达到统计学显著结果，但测量值接近显著性水平。鼓室内注射地塞米松在顺铂耳毒性后采用不同剂量和应用方案可能具有显著的治疗效果。
