Wu Qiu Jue, Zhou Yan Min, Wu Ya Nan, Zhang Li Li, Wang Tian
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, Henan, PR China.
Vet Immunol Immunopathol. 2013 May 15;153(1-2):70-6. doi: 10.1016/j.vetimm.2013.02.006. Epub 2013 Feb 13.
The protection of intestinal barrier function and the anti-inflammatory effects of natural clinoptilolite (NCLI) and modified clinoptilolite (MCLI) were investigated in broilers that were repeatedly challenged with lipopolysaccharide (LPS). A total of 288 1-d-old broiler chicks were divided equally into three treatment groups: control, NCLI-treated (2%) and MCLI-treated (2%). Half of the birds from each treatment group were challenged with 0.9% NaCl solution or LPS (250μg/kg body weight, administered orally) at 16, 18 and 21d of age. The results indicated that, prior to LPS challenge, the diet had no effect on bird growth performance (P>0.05). The oral administration of LPS was also not associated with any significant changes in poultry performance (P>0.05). In LPS-challenged birds that were pretreated with NCLI (2%) or MCLI (2%), the LPS-induced increases in the plasma and intestinal mucosa concentrations of TNF-α, IL-1β, IL-2, IL-6, IL-4 and IL-10 were dramatically attenuated. Additionally, significant decreases in the plasma d-lactic acid and diamine oxidase (DAO) levels were found in birds that were pretreated with NCLI or MCLI. Furthermore, both NCLI and MCLI reduced the sICAM-1 concentration in the intestinal mucosa. In conclusion, NCLI and MCLI are able to prevent the LPS-induced intestinal mucosa damage and inflammatory response in vivo. These beneficial effects suggest that NCLI and MCLI act as anti-inflammatory agents in part by inhibiting neutrophil infiltration and hyperactivation and by suppressing the secretion of various plasma and intestinal mucosa inflammatory mediators.
在反复用脂多糖（LPS）攻击的肉鸡中，研究了天然斜发沸石（NCLI）和改性斜发沸石（MCLI）对肠道屏障功能的保护作用及抗炎作用。将288只1日龄肉鸡平均分为三个处理组：对照组、NCLI处理组（2%）和MCLI处理组（2%）。每个处理组的一半鸡在16、18和21日龄时分别用0.9%氯化钠溶液或LPS（250μg/kg体重，口服）进行攻击。结果表明，在LPS攻击前，日粮对鸡的生长性能没有影响（P>0.05）。口服LPS也与家禽生产性能的任何显著变化无关（P>0.05）。在用NCLI（2%）或MCLI（2%）预处理的LPS攻击鸡中，LPS诱导的血浆和肠黏膜中TNF-α、IL-1β、IL-2、IL-6、IL-4和IL-10浓度的升高显著减弱。此外，在用NCLI或MCLI预处理的鸡中，血浆d-乳酸和二胺氧化酶（DAO）水平显著降低。此外，NCLI和MCLI均降低了肠黏膜中sICAM-1的浓度。总之，NCLI和MCLI能够预防LPS诱导的体内肠黏膜损伤和炎症反应。这些有益作用表明，NCLI和MCLI作为抗炎剂，部分是通过抑制中性粒细胞浸润和过度活化以及抑制各种血浆和肠黏膜炎症介质的分泌来发挥作用的。
