Xu Huiping, Gong Lu, Zhang Xiaodan, Li Zhenyi, Fu Jianyang, Lv Zengpeng, Guo Yuming
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Poult Sci. 2025 Feb;104(2):104676. doi: 10.1016/j.psj.2024.104676. Epub 2024 Dec 28.
This study investigated the optimal tannic acid dosage and assessed tolerance levels in broiler chickens. In experiment 1, 525 broilers were randomly divided into 5 treatment groups, the control group (CON group) and groups TA1 to TA4, corresponding to treatments of 0.025, 0.05, 0.075, and 0.1 % tannic acid, respectively, to determine the effect of tannic acid on broiler growth performance and gut health. Experiment 2 was performed to evaluate the tolerance of tannic acid; 416 broilers were randomly divided into control (CTR), 0.075 % tannic acid (TA), 0.375 % tannic acid (5TA), and 0.75 % tannic acid (10TA) groups. In the first experiment, compared with that in the CON group, the growth performance and the ileal intestinal villi height to crypt depth ratio showed a quadratic curve increase with tannic acid supplementation (P < 0.05). Adding 0.05 % to 0.075 % tannic acid significantly improved the growth performance, intestinal morphology, and intestinal barrier function (P < 0.05). Tannic acid concentrations of 0.075 % significantly increased the abundance of Firmicutes and Lactobacillaceae in the ileum and decreased the abundance of Vibrionaceae and Yersiniaceae (P < 0.05). In experiment 2, the growth performance of the TA group significantly improved compared with that of the CTR group (P < 0.05). The F/G was significantly higher in the 5TA and 10TA groups than in the CTR group (P < 0.05), and the 10TA group had significantly reduced body weight on d 21 (P < 0.05). The addition of tannic acid resulted in significant glomerular and glandular hyperplasia, as well as muscularis thickening of the gizzard mucosa. However, broilers could not tolerate tannic acid doses of 0.375 % and above. Tannic acid supplementation may protect the proventriculus mucosal layer, reduce villi atrophy, and enhance growth performance by positively influencing the intestinal microbiota, villus morphology, and intestinal barrier function.
本研究调查了肉仔鸡的最佳单宁酸剂量并评估了其耐受水平。在实验1中，525只肉仔鸡被随机分为5个处理组，即对照组（CON组）和TA1至TA4组，分别对应0.025%、0.05%、0.075%和0.1%的单宁酸处理，以确定单宁酸对肉仔鸡生长性能和肠道健康的影响。进行实验2以评估单宁酸的耐受性；416只肉仔鸡被随机分为对照组（CTR）、0.075%单宁酸组（TA）、0.375%单宁酸组（5TA）和0.75%单宁酸组（10TA）。在第一个实验中，与CON组相比，随着单宁酸添加量的增加，生长性能和回肠肠绒毛高度与隐窝深度比呈二次曲线增加（P<0.05）。添加0.05%至0.075%的单宁酸显著改善了生长性能、肠道形态和肠道屏障功能（P<0.05）。0.075%的单宁酸浓度显著增加了回肠中厚壁菌门和乳酸杆菌科的丰度，并降低了弧菌科和耶尔森菌科的丰度（P<0.05）。在实验2中，TA组的生长性能与CTR组相比显著提高（P<0.05）。5TA组和10TA组的料重比显著高于CTR组（P<0.05），并且10TA组在第21天时体重显著降低（P<0.05）。添加单宁酸导致腺胃和肌胃黏膜的显著增生以及肌层增厚。然而，肉仔鸡无法耐受0.375%及以上剂量的单宁酸。补充单宁酸可能通过积极影响肠道微生物群、绒毛形态和肠道屏障功能来保护腺胃黏膜层、减少绒毛萎缩并提高生长性能。
