Shou Qi-Yang, Fu Hui-Ying, Zhang Li-Zong, Cai Yue-Qin, Chen Fang-Ming, Chen Min-Li
Animal Laboratory Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Zhongguo Zhong Yao Za Zhi. 2012 Dec;37(23):3618-23.
To study and discuss the effect and mechanism of Hirsutella sinensis mycelium (HSM) on idiopathic pulmonary fibrosis in rats.
Forty Wistar rats were divided into five groups: the normal control group, the model control group, the high-dose group (1.0 g x kg(-1) HSM), the low-dose group (0.5 g x kg(-1) HSM), and the positive control group (10 mg x kg(-1) hydrocortisone). In addition to rats in the normal control group, the pulmonary fibrosis model was established by injecting 5 mg x kg(-1) bleomycin into rat tracheas for consecutively 28 days, in order to observe their lung function, lung tissue hydroxyproline, cytokines and pathology.
After rats were administered with HSM, 0.5 g x kg(-1) and 1.0 g x kg(-1) HSM could significantly decrease lung index and hydroxyproline content (P<0.01), while notably improving pulmonary function, alveolus inflammation and fibrosis degree (P<0.05, P<0.01); 1.0 g x kg(-1) HSM could decrease significantly protein expressions of TNF-alpha, IL-1beta and TGF-beta1 in lung tissues, while increasing significantly protein expressions of IFN-gamma (P<0.05).
HSM have better effect in treating idiopathic pulmonary fibrosis in rats. Its treatment effect and mechanism are related to the regulation of TNF-alpha, IL-1beta and TGF-beta1 and IFN-gamma imbalance.
研究和探讨中华被毛孢菌丝体（HSM）对大鼠特发性肺纤维化的作用及机制。
将40只Wistar大鼠分为五组：正常对照组、模型对照组、高剂量组（1.0 g·kg⁻¹ HSM）、低剂量组（0.5 g·kg⁻¹ HSM）和阳性对照组（10 mg·kg⁻¹氢化可的松）。除正常对照组大鼠外，其余大鼠连续28天经气管注射5 mg·kg⁻¹博来霉素建立肺纤维化模型，观察其肺功能、肺组织羟脯氨酸、细胞因子及病理变化。
给予HSM后，0.5 g·kg⁻¹和1.0 g·kg⁻¹ HSM可显著降低肺指数和羟脯氨酸含量（P<0.01），同时显著改善肺功能、肺泡炎症及纤维化程度（P<0.05，P<0.01）；1.0 g·kg⁻¹ HSM可显著降低肺组织中TNF-α、IL-1β和TGF-β1的蛋白表达，同时显著增加IFN-γ的蛋白表达（P<0.05）。
HSM对大鼠特发性肺纤维化有较好的治疗作用。其治疗作用及机制与调节TNF-α、IL-1β、TGF-β1和IFN-γ失衡有关。
