Department of Pathobiology key lab of Ministry of Education, Norman Bethune College of Medicine, Jilin University, China and Department of Sports Science, Faculty of Physical Education, Suez Canal University, Egypt.
J Hum Kinet. 2012 Dec;35:69-79. doi: 10.2478/v10078-012-0080-y. Epub 2012 Dec 30.
Exercise is one of the most powerful non-pharmacological strategies, which can affect nearly all cells and organs in the body. Changes in the behavior of adult stem cells have been shown to occur in response to exercise. Exercise may act on regenerative potential of tissues by altering the ability to generate new stem cells and differentiated cells that are able to carry out tissue specific functions. The purpose of this study was to reveal the role of aerobic and anaerobic training programs on CD34+ Stem Cells and chosen physiological variables. Twenty healthy male athletes aged 18-24 years were recruited for this study. Healthy low active males and BMI matched participants (n=10) aged 20-22 years were recruited as controls. Aerobic and anaerobic training programs for 12 weeks were conducted. VO2max pulse observation was carried out using the Astrand Rhyming protocol. RBCs, WBCs, HB and hematocrit were estimated using a coulter counter, lactate by the Accusport apparatus, CD34+ stem cells by flow cytometry. VO2max was increased significantly in case of the aerobic training program compared to anaerobic one (62±2.2 ml/kg/min vs. 54±2.1 ml/kg/min). Haemotological values increased significantly in the anaerobic program when compared to the aerobic one, RBCs (5.3±0.3 and 4.9±0.2 mln/ul), WBCs (6.6±0.5 and 6.1±0.4 thous/ul), HB (15.4±0.4 and 14.2±0.5 g/de), Hematocrit (4.6±1.2 and 4.4±1.1 %), CD34+ stem cells count increased significantly in case of the anaerobic program compared to the aerobic (251.6±21.64 and 130±14.61) and sedentary one (172±24.10). These findings suggest that anaerobic training programs provoke better adaptation to exercise and stem cell counts may differ between trained and sedentary subjects. Circulating immature cells are likely to be involved in angiogenesis and repair process, both mechanisms being associated with strenuous exercise. Knowledge of the physiological effects of training on stem cells might be of potential clinical use.
运动是最强大的非药物治疗策略之一，可以影响身体几乎所有的细胞和器官。已经证明，运动可以改变成年干细胞的行为，从而产生新的干细胞和能够执行组织特定功能的分化细胞。本研究的目的是揭示有氧和无氧训练方案对 CD34+干细胞和选定生理变量的作用。招募了 20 名年龄在 18-24 岁的健康男性运动员进行这项研究。招募了年龄在 20-22 岁的健康低活动男性和 BMI 匹配的参与者（n=10）作为对照组。进行了 12 周的有氧和无氧训练计划。使用 Astrand 韵律方案进行 VO2max 脉搏观察。使用库尔特计数器估计 RBC、WBC、HB 和血细胞比容，使用 Accusport 仪器测量乳酸，使用流式细胞术测量 CD34+干细胞。与无氧训练方案相比，有氧训练方案显著提高了 VO2max（62±2.2 ml/kg/min 与 54±2.1 ml/kg/min）。与有氧训练方案相比，无氧训练方案中的血液学值显著增加，RBC（5.3±0.3 和 4.9±0.2 mln/ul）、WBC（6.6±0.5 和 6.1±0.4 thous/ul）、HB（15.4±0.4 和 14.2±0.5 g/de）、Hct（4.6±1.2 和 4.4±1.1%），CD34+干细胞计数在无氧训练方案中比有氧训练方案（251.6±21.64 和 130±14.61）和久坐不动方案（172±24.10）显著增加。这些发现表明，无氧训练方案可以更好地适应运动，并且训练和久坐不动的个体之间的干细胞计数可能不同。循环幼稚细胞可能参与血管生成和修复过程，这两种机制都与剧烈运动有关。了解训练对干细胞的生理影响可能具有潜在的临床应用价值。
