Hashimoto Y, Maxam A M, Greene M I
Department of Pathology, University of Pennsylvania, Philadelphia 19104.
Nucleic Acids Res. 1990 May 25;18(10):3027-33. doi: 10.1093/nar/18.10.3027.
We have determined the DNA sequences in the J2-C2 intron of the T cell receptor (TCR) beta gene and analyzed nuclear proteins binding to this region. Previously, we identified two tissue-specific DNase I hypersensitive regions, potential regulatory regions, in the J-C intron. The DNA sequence of the J2-C2 intron revealed that both DNase I hypersensitive regions have similar DNA sequences, suggesting that these regions are evolutionarily conserved. We have also identified tissue-specific nuclear-protein binding regions downstream of the DNase hypersensitive regions. Although transcriptional enhancer activity was not observed in the hypersensitive regions or the adjacent protein binding regions in the J-C intron, our findings suggest that the TCR-beta J-C intron may contain some other type of regulatory element.
我们已经测定了T细胞受体（TCR）β基因J2-C2内含子中的DNA序列，并分析了与该区域结合的核蛋白。此前，我们在J-C内含子中鉴定出两个组织特异性的DNA酶I高敏感区域，即潜在的调控区域。J2-C2内含子的DNA序列显示，这两个DNA酶I高敏感区域具有相似的DNA序列，表明这些区域在进化上是保守的。我们还在DNA酶高敏感区域下游鉴定出了组织特异性的核蛋白结合区域。尽管在J-C内含子的高敏感区域或相邻的蛋白结合区域未观察到转录增强子活性，但我们的研究结果表明，TCR-β J-C内含子可能包含一些其他类型的调控元件。
