Hall R M, Stokes H W
CSIRO Division of Biotechnology, NSW, Australia.
Plasmid. 1990 Jan;23(1):76-9. doi: 10.1016/0147-619x(90)90047-g.
A family of novel potentially mobile DNA elements called integrons, has recently been described (H. W. Stokes and R. M. Hall, 1989, Mol. Microbiol. 3, 1669-1683). The integrons present in the plasmids pDGO100 and pSa are unusual in that they include a duplication of the sulI gene which is located in one of the two conserved segments that make up these elements. In order to define the nature of the duplication in pDGO100, we have sequenced the sulI gene region located between the aadB and the dhfr genes of pDGO100. This region includes the first 1355 bases of the 2026-base 3'-conserved segment present in the integrons of Tn21, R46 and R388, and the sequence identity in pDGO100 ceases 24 bases beyond the end of the sulI gene. This position corresponds to the center of a 59-base element, a remnant of which is located at the end of sulI. This finding suggests that the 59-base element may have been involved in the event which gave rise to the partial duplication.
最近描述了一类称为整合子的新型潜在可移动DNA元件家族（H. W. 斯托克斯和R. M. 霍尔，1989年，《分子微生物学》3，1669 - 1683）。存在于质粒pDGO100和pSa中的整合子不同寻常之处在于，它们包含sulI基因的一个重复序列，该基因位于构成这些元件的两个保守区段之一中。为了确定pDGO100中重复序列的性质，我们对位于pDGO100的aadB和dhfr基因之间的sulI基因区域进行了测序。该区域包括Tn21、R46和R388整合子中2026个碱基的3' - 保守区段的前1355个碱基，并且pDGO100中的序列一致性在sulI基因末端之后24个碱基处终止。该位置对应于一个59个碱基元件的中心，其残余部分位于sulI末端。这一发现表明，59个碱基的元件可能参与了导致部分重复的事件。
