Photonics Group, Department of Physics, Imperial College London, London, UK.
Opt Lett. 2013 Mar 15;38(6):851-3. doi: 10.1364/OL.38.000851.
We describe an angular multiplexing technique for optical projection tomography that improves resolution, signal-to-noise ratio, and imaging speed by ameliorating the trade-off between spatial resolution and depth of field and improving the light collection efficiency. Here we demonstrate that imaging at two orthogonal angular projections simultaneously, focused on shifted planes in the sample, improves the average spatial resolution by ~20% and the light collection efficiency by a factor of ~4, thereby enabling increased acquisition speed and reduced light dose.
我们描述了一种用于光学投影层析成像的角度复用技术，通过改善空间分辨率和景深之间的权衡，并提高光收集效率，从而提高分辨率、信噪比和成像速度。在这里，我们证明了同时在两个正交角度投影下成像，聚焦在样品中的移位平面上，可以将平均空间分辨率提高约 20%，并将光收集效率提高约 4 倍，从而实现更快的采集速度和更低的光剂量。
