Laboratory of Translational Therapeutics, Pharmacology Research Center, Bio-Organic Science Division, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong-gu, Daejeon 305-600, Republic of Korea.
Evid Based Complement Alternat Med. 2013;2013:748687. doi: 10.1155/2013/748687. Epub 2013 Feb 10.
Antlers have been traditionally used for thousands of years as a natural product with medicinal and pharmaceutical properties. In developing healthy foods, Bacillus-mediated fermentation is widely used to enhance the biological activity of nutrients in foods. Recently, fermentation was shown to enhance the osteogenic activity of antlers. This study aimed to elucidate the antiresorptive activity of Bacillus-fermented antler and its mode of action. We found that Bacillus-fermented antler extract strongly inhibited osteoclast differentiation by downregulating the expression and activity of nuclear factor of activated T cells, cytoplasmic 1 (NFATc1). This extract also inhibited the activation of phospholipase C γ 2 (PLC γ 2), a signaling molecule that could regulate NFATc1 transcriptional activity. This suggested that Bacillus-fermented antler extract could inhibit PLC γ 2-NFATc1 signaling required for bone resorption and cell fusion. Consequently, Bacillus-fermented antler extract might benefit osteoclast-related disorders, including osteoporosis; furthermore, it may improve gastrointestinal activity.
鹿茸作为一种具有药用和药物特性的天然产品，已经被传统使用了数千年。在开发健康食品方面，芽孢杆菌介导的发酵被广泛用于增强食品中营养物质的生物活性。最近的研究表明，发酵可以增强鹿茸的成骨活性。本研究旨在阐明芽孢杆菌发酵鹿茸的抗吸收活性及其作用机制。我们发现，芽孢杆菌发酵鹿茸提取物通过下调激活 T 细胞核因子，细胞质 1（NFATc1）的表达和活性，强烈抑制破骨细胞分化。该提取物还抑制磷脂酶 Cγ2（PLCγ2）的激活，PLCγ2 是一种可以调节 NFATc1 转录活性的信号分子。这表明芽孢杆菌发酵鹿茸提取物可以抑制破骨细胞相关的骨吸收和细胞融合所必需的 PLCγ2-NFATc1 信号通路。因此，芽孢杆菌发酵鹿茸提取物可能有益于与破骨细胞相关的疾病，包括骨质疏松症；此外，它可能改善胃肠道活动。
