脲酶金属中心的生物合成。
Biosynthesis of the urease metallocenter.
机构信息
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA.
出版信息
J Biol Chem. 2013 May 10;288(19):13178-85. doi: 10.1074/jbc.R112.446526. Epub 2013 Mar 28.
Abstract
Metalloenzymes often require elaborate metallocenter assembly systems to create functional active sites. The medically important dinuclear nickel enzyme urease provides an excellent model for studying metallocenter assembly. Nickel is inserted into the urease active site in a GTP-dependent process with the assistance of UreD/UreH, UreE, UreF, and UreG. These accessory proteins orchestrate apoprotein activation by delivering the appropriate metal, facilitating protein conformational changes, and possibly providing a requisite post-translational modification. The activation mechanism and roles of each accessory protein in urease maturation are the subject of ongoing studies, with the latest findings presented in this minireview.
摘要
金属酶通常需要精心设计的金属中心组装系统来创建功能性的活性位点。医学上重要的双核镍酶脲酶为研究金属中心组装提供了一个极好的模型。镍在 GTP 依赖性过程中被插入脲酶活性部位，辅助蛋白为 UreD/UreH、UreE、UreF 和 UreG。这些辅助蛋白通过递呈合适的金属、促进蛋白质构象变化，并可能提供必要的翻译后修饰，来协调脱辅基蛋白的激活。脲酶成熟过程中每种辅助蛋白的激活机制和作用是正在进行的研究课题，本综述介绍了最新的发现。
相似文献
1
Biosynthesis of the urease metallocenter.脲酶金属中心的生物合成。
J Biol Chem. 2013 May 10;288(19):13178-85. doi: 10.1074/jbc.R112.446526. Epub 2013 Mar 28.
2
GTP-dependent activation of urease apoprotein in complex with the UreD, UreF, and UreG accessory proteins.与UreD、UreF和UreG辅助蛋白形成复合物的脲酶脱辅基蛋白的GTP依赖性激活。
Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11140-4. doi: 10.1073/pnas.96.20.11140.
3
Structural insights into how GTP-dependent conformational changes in a metallochaperone UreG facilitate urease maturation.金属伴侣蛋白 UreG 中 GTP 依赖性构象变化促进脲酶成熟的结构见解。
Proc Natl Acad Sci U S A. 2017 Dec 19;114(51):E10890-E10898. doi: 10.1073/pnas.1712658114. Epub 2017 Dec 4.
4
Mutational and Computational Evidence That a Nickel-Transfer Tunnel in UreD Is Used for Activation of Klebsiella aerogenes Urease.关于产气克雷伯菌脲酶激活过程中，脲酶激活蛋白UreD中镍转运通道发挥作用的突变及计算证据。
Biochemistry. 2015 Oct 20;54(41):6392-401. doi: 10.1021/acs.biochem.5b00942. Epub 2015 Oct 5.
5
UreE stimulation of GTP-dependent urease activation in the UreD-UreF-UreG-urease apoprotein complex.在UreD-UreF-UreG-脲酶脱辅基蛋白复合物中，UreE对GTP依赖性脲酶激活的刺激作用。
Biochemistry. 2000 Oct 10;39(40):12435-40. doi: 10.1021/bi001296o.
6
Structure of UreG/UreF/UreH complex reveals how urease accessory proteins facilitate maturation of Helicobacter pylori urease.UreG/UreF/UreH 复合物的结构揭示了脲酶辅助蛋白如何促进幽门螺杆菌脲酶的成熟。
PLoS Biol. 2013 Oct;11(10):e1001678. doi: 10.1371/journal.pbio.1001678. Epub 2013 Oct 8.
7
Klebsiella aerogenes UreF: identification of the UreG binding site and role in enhancing the fidelity of urease activation.产酸克雷伯氏菌 UreF：鉴定 UreG 结合位点及其在增强脲酶激活保真度中的作用。
Biochemistry. 2012 Mar 20;51(11):2298-308. doi: 10.1021/bi3000897. Epub 2012 Mar 6.
8
In vitro activation of urease apoprotein and role of UreD as a chaperone required for nickel metallocenter assembly.脲酶脱辅基蛋白的体外激活以及UreD作为镍金属中心组装所需伴侣蛋白的作用。
Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3233-7. doi: 10.1073/pnas.91.8.3233.
9
Klebsiella aerogenes urease gene cluster: sequence of ureD and demonstration that four accessory genes (ureD, ureE, ureF, and ureG) are involved in nickel metallocenter biosynthesis.产气克雷伯菌脲酶基因簇：ureD序列及四个辅助基因（ureD、ureE、ureF和ureG）参与镍金属中心生物合成的证明
J Bacteriol. 1992 Jul;174(13):4324-30. doi: 10.1128/jb.174.13.4324-4330.1992.
10
Evidence for the presence of urease apoprotein complexes containing UreD, UreF, and UreG in cells that are competent for in vivo enzyme activation.在具有体内酶激活能力的细胞中存在含有脲酶脱辅基蛋白复合物（UreD、UreF和UreG）的证据。
J Bacteriol. 1995 Apr;177(8):1947-51. doi: 10.1128/jb.177.8.1947-1951.1995.
引用本文的文献
1
New Directions in Urease Inhibitors: Focusing on Nickel Ions Transfer and Auxiliary Protein Interactions During Urease Maturation.脲酶抑制剂的新方向：聚焦于脲酶成熟过程中的镍离子转移和辅助蛋白相互作用
Infect Drug Resist. 2025 Jun 17;18:3037-3053. doi: 10.2147/IDR.S519194. eCollection 2025.
2
Bacterial Metallostasis: Metal Sensing, Metalloproteome Remodeling, and Metal Trafficking.细菌金属稳态：金属感应、金属蛋白质组重塑及金属转运
Chem Rev. 2024 Dec 25;124(24):13574-13659. doi: 10.1021/acs.chemrev.4c00264. Epub 2024 Dec 10.
3
Protein interactions in human pathogens revealed through deep learning.通过深度学习揭示人类病原体中的蛋白质相互作用。
Nat Microbiol. 2024 Oct;9(10):2642-2652. doi: 10.1038/s41564-024-01791-x. Epub 2024 Sep 18.
4
UreR coordinates cellular functions required for urease activity.UreR 协调脲酶活性所需的细胞功能。
J Bacteriol. 2024 Apr 18;206(4):e0003124. doi: 10.1128/jb.00031-24. Epub 2024 Mar 27.
5
Mitochondrial Function in Health and Disease: Responses to Helicobacter pylori Metabolism and Impact in Gastric Cancer Development.线粒体功能与健康和疾病：幽门螺杆菌代谢的反应及其对胃癌发展的影响。
Curr Top Microbiol Immunol. 2023;444:53-81. doi: 10.1007/978-3-031-47331-9_3.
6
Five decades of metalloenzymology.五十载金属酶学研究。
Enzymes. 2023;54:71-105. doi: 10.1016/bs.enz.2023.03.001. Epub 2023 Mar 30.
7
Growth Increase in the Herbaceous Plant by the Plant Growth-Promoting Rhizobacteria HyangYak-01.植物促生根际细菌HyangYak - 01对草本植物生长的促进作用
Plants (Basel). 2023 Jun 21;12(13):2398. doi: 10.3390/plants12132398.
8
Delivering a toxic metal to the active site of urease.将有毒金属递送到脲酶的活性部位。
Sci Adv. 2023 Apr 21;9(16):eadf7790. doi: 10.1126/sciadv.adf7790.
9
Urease of Aspergillus fumigatus Is Required for Survival in Macrophages and Virulence.烟曲霉脲酶是其在巨噬细胞内存活和致病所必需的。
Microbiol Spectr. 2023 Mar 14;11(2):e0350822. doi: 10.1128/spectrum.03508-22.
10
Understanding and application of Bacillus nitrogen regulation: A synthetic biology perspective.理解和应用芽孢杆菌氮调控：合成生物学视角。
J Adv Res. 2023 Jul;49:1-14. doi: 10.1016/j.jare.2022.09.003. Epub 2022 Sep 12.
本文引用的文献
1
Opinion: nickel and urease in plants: still many knowledge gaps.观点：植物中的镍和脲酶：仍有许多知识空白。
Plant Sci. 2013 Feb;199-200:79-90. doi: 10.1016/j.plantsci.2012.10.010. Epub 2012 Nov 10.
2
Structure of the proton-gated urea channel from the gastric pathogen Helicobacter pylori.质子门控尿素通道结构来自胃病原体幽门螺旋杆菌。
Nature. 2013 Jan 10;493(7431):255-8. doi: 10.1038/nature11684. Epub 2012 Dec 9.
3
Specific metal recognition in nickel trafficking.特定金属在镍运输中的识别。
Biochemistry. 2012 Oct 9;51(40):7816-32. doi: 10.1021/bi300981m. Epub 2012 Sep 28.
4
Evidence-based docking of the urease activation complex.基于证据的尿素酶激活复合物对接。
J Biomol Struct Dyn. 2013;31(8):854-61. doi: 10.1080/07391102.2012.713782. Epub 2012 Sep 10.
5
Helicobacter pylori hydrogenase accessory protein HypA and urease accessory protein UreG compete with each other for UreE recognition.幽门螺杆菌氢化酶辅助蛋白HypA和脲酶辅助蛋白UreG相互竞争以识别UreE。
Biochim Biophys Acta. 2012 Oct;1820(10):1519-25. doi: 10.1016/j.bbagen.2012.06.002. Epub 2012 Jun 12.
6
Klebsiella aerogenes UreF: identification of the UreG binding site and role in enhancing the fidelity of urease activation.产酸克雷伯氏菌 UreF：鉴定 UreG 结合位点及其在增强脲酶激活保真度中的作用。
Biochemistry. 2012 Mar 20;51(11):2298-308. doi: 10.1021/bi3000897. Epub 2012 Mar 6.
7
Biochemical and structural studies on native and recombinant Glycine max UreG: a detailed characterization of a plant urease accessory protein.大豆尿酶辅助蛋白的天然和重组 Glycine max UreG 的生化和结构研究：一种植物尿酶辅助蛋白的详细特征描述。
Plant Mol Biol. 2012 Mar;78(4-5):461-75. doi: 10.1007/s11103-012-9878-1. Epub 2012 Jan 22.
8
Apoprotein isolation and activation, and vibrational structure of the Helicobacter mustelae iron urease.幽门螺旋杆菌铁尿素酶的载脂蛋白分离与激活，以及振动结构。
J Inorg Biochem. 2012 Jun;111:195-202. doi: 10.1016/j.jinorgbio.2011.10.016. Epub 2011 Nov 28.
9
Unraveling the Helicobacter pylori UreG zinc binding site using X-ray absorption spectroscopy (XAS) and structural modeling.运用 X 射线吸收光谱学（XAS）和结构建模技术揭示幽门螺杆菌 UreG 的锌结合位点。
J Biol Inorg Chem. 2012 Mar;17(3):353-61. doi: 10.1007/s00775-011-0857-9. Epub 2011 Nov 9.
10
Assembly of preactivation complex for urease maturation in Helicobacter pylori: crystal structure of UreF-UreH protein complex.幽门螺杆菌脲酶成熟前激活复合物的组装：UreF-UreH 蛋白复合物的晶体结构。
J Biol Chem. 2011 Dec 16;286(50):43241-9. doi: 10.1074/jbc.M111.296830. Epub 2011 Oct 19.
Zotero 插件