Tuaillon N, Muller S, Pasquali J L, Bordigoni P, Youinou P, Van Regenmortel M H
Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.
Int Arch Allergy Appl Immunol. 1990;91(3):297-305. doi: 10.1159/000235131.
Antihistone antibodies were detected in the sera of a randomly selected group of patients with rheumatoid arthritis (RA) and juvenile chronic arthritis (JCA) by an enzyme-linked immunosorbent assay (ELISA) with the five individual histones and by immunoblotting. In ELISA, the overall frequency of antihistone antibodies in RA and JCA was 51 and 44%, respectively. Antibodies present in these serum samples were further studied by ELISA by means of 17 core histone synthetic peptides. The fragment 1-21 of H3 was recognized by 60% of RA sera and by 62% of JCA sera. In addition, at least four terminal or internal peptides of H3 and H4 were recognized by more than a third of JCA sera, while only two of these peptides reacted with 20% of RA sera. Many of the sera that did not show any reactivity with the whole histone reacted with various histone peptides. This finding demonstrates the usefulness of synthetic peptides for identifying autoantibodies.
采用含有五种单个组蛋白的酶联免疫吸附测定（ELISA）和免疫印迹法，在一组随机挑选的类风湿性关节炎（RA）和青少年慢性关节炎（JCA）患者的血清中检测抗组蛋白抗体。在ELISA中，RA和JCA中抗组蛋白抗体的总体频率分别为51%和44%。通过17种核心组蛋白合成肽，采用ELISA对这些血清样本中存在的抗体作进一步研究。H3的1-21片段被60%的RA血清和62%的JCA血清识别。此外，超过三分之一的JCA血清识别H3和H4的至少四种末端或内部肽段，而这些肽段中只有两种与20%的RA血清发生反应。许多与完整组蛋白无任何反应性的血清与各种组蛋白肽发生反应。这一发现证明了合成肽在鉴定自身抗体方面的实用性。
