Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, Hong Kong SAR, China.
IEEE Trans Ultrason Ferroelectr Freq Control. 2013 Apr;60(4):718-26. doi: 10.1109/TUFFC.2013.2620.
Zebrafish can fully regenerate their myocardium after ventricular resection without evidence of scars. This extraordinary regenerative ability provides an excellent model system to study the activation of the regenerative potential for human heart tissue. In addition to the morphology, it is vital to understand the cardiac function of zebrafish. To characterize adult zebrafish cardiac function, an ultrasound biomicroscope (UBM) was customized for real-time imaging of the zebrafish heart (about 1 mm in diameter) at a resolution of around 37 μm. Moreover, we developed an image segmentation algorithm to track the cardiac boundary and measure the dynamic size of the zebrafish heart for further quantification of zebrafish cardiac function. The effectiveness and accuracy of the proposed segmentation algorithm were verified on a tissuemimicking phantom and in vivo zebrafish echocardiography. The quantitative evaluation demonstrated that the accuracy of the proposed algorithm is comparable to the manual delineation by experts.
斑马鱼在心室切除后可以完全再生心肌，没有疤痕的证据。这种非凡的再生能力为研究人类心脏组织的再生潜能提供了一个极好的模型系统。除了形态学，了解斑马鱼的心脏功能也至关重要。为了描述成年斑马鱼的心脏功能，我们专门为实时成像定制了一个超声生物显微镜 (UBM)，可以以大约 37 微米的分辨率对直径约为 1 毫米的斑马鱼心脏进行成像。此外，我们开发了一种图像分割算法来跟踪心脏边界并测量斑马鱼心脏的动态大小，以便进一步量化斑马鱼的心脏功能。所提出的分割算法的有效性和准确性已在组织模拟体和体内斑马鱼超声心动图中得到验证。定量评估表明，该算法的准确性可与专家的手动勾画相媲美。
