评价 NucliSENS EasyQ KPC 检测试剂盒用于检测产碳青霉烯酶肠杆菌科细菌。
Evaluation of the NucliSENS EasyQ KPC assay for detection of Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae.
机构信息
Manchester Royal Infirmary, Manchester, United Kingdom.
出版信息
J Clin Microbiol. 2013 Jun;51(6):1948-50. doi: 10.1128/JCM.00057-13. Epub 2013 Apr 3.
Abstract
The NucliSENS EasyQ KPC assay (bioMérieux SA, Marcy l'Etoile, France) was compared with a routinely used phenotypic method for detection of Enterobacteriaceae producing Klebsiella pneumoniae carbapenemase (KPC)-type carbapenemases, using 806 stool samples and rectal swabs. Compared with the phenotypic method, the EasyQ KPC assay had a sensitivity and specificity of 93.3% and 99.0%, respectively, in this setting, with diverse KPC producers not limited to ST258 Klebsiella pneumoniae.
摘要
采用诺利思易检 KPC 检测试剂盒（bioMérieux SA，Marcy l'Etoile，法国）与常规表型方法对 806 份粪便样本和直肠拭子进行比较，用于检测产肺炎克雷伯菌碳青霉烯酶（KPC）型碳青霉烯酶的肠杆菌科。在这种情况下，与表型方法相比，易检 KPC 检测试剂盒的灵敏度和特异性分别为 93.3%和 99.0%，检测的产 KPC 菌不仅限于 ST258 型肺炎克雷伯菌。
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2
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