Kobayashi N, Hamamoto Y, Yamamoto N
Department of Virology and Parasitology, School of Medicine Yamaguchi University, Japan.
Med Microbiol Immunol. 1990;179(2):115-22. doi: 10.1007/BF00198532.
The production of tumor necrosis factor (TNF)-alpha and TNF-beta by various human hematopoietic cell lines was quantitatively examined using a highly sensitive radioimmunoassay specific to TNF-alpha, or a cytolytic assay performed with mouse L929 cells. It was found that the HTLV-1-infected T cell lines examined produced large amounts of both TNF-alpha and TNF-beta. In particular, interleukin-2 (IL-2)-dependent cell lines produced large amounts of TNF-alpha. In contrast, human cell lines not infected with HTLV-1 essentially did not produce either of the TNFs. It was also found that the high production of TNF-alpha by HTLV-1-infected cells partially correlated to their high sensitivity to human immunodeficiency virus (HIV) infection. Treatment of MT-4 cells, one of the most HIV-sensitive HTLV-1-infected cell lines, with antibody specific to TNF-alpha reduced their sensitivity to HIV infection.
使用对肿瘤坏死因子-α（TNF-α）特异的高灵敏度放射免疫测定法，或用小鼠L929细胞进行的细胞溶解测定法，对多种人类造血细胞系产生TNF-α和TNF-β的情况进行了定量检测。结果发现，所检测的感染人嗜T淋巴细胞病毒1型（HTLV-1）的T细胞系能产生大量的TNF-α和TNF-β。特别是，依赖白细胞介素-2（IL-2）的细胞系能产生大量的TNF-α。相比之下，未感染HTLV-1的人类细胞系基本上不产生这两种TNF。还发现，感染HTLV-1的细胞对TNF-α的高产量与其对人类免疫缺陷病毒（HIV）感染的高敏感性部分相关。用针对TNF-α的抗体处理MT-4细胞（最易感染HIV的HTLV-1感染细胞系之一），可降低其对HIV感染的敏感性。
