College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Int J Syst Evol Microbiol. 2013 Oct;63(Pt 10):3563-3567. doi: 10.1099/ijs.0.050971-0. Epub 2013 Apr 5.
A novel extremely halophilic archaeon KCY07-B2(T) was isolated from a salt mine in Kuche county, Xinjiang province, China. Colonies were cream-pigmented and cells were pleomorphic rod-shaped. Strain KCY07-B2(T) was able to grow at 25-50 °C (optimum 37-45 °C) and pH 6.0-8.0 (optimum 7.0). The strain required at least 1.9 M NaCl for growth. MgCl2 was not required. Cells lysed in distilled water. Polar lipid analysis revealed the presence of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, derived from both C20C20 and C20C25 glycerol diethers, together with five glyolipids. The bis-sulfated glycolipid S2-DGD-1 was present. The DNA G+C content was 62.5 mol%. Analysis of the 16S rRNA gene sequence revealed that strain KCY07-B2(T) was closely related to Halopiger xanaduensis SH-6(T) and Halopiger aswanensis 56(T) (95.8 % and 95.5 % similarity, respectively). On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain KCY07-B2(T) is considered to represent a novel species of the genus Halopiger, for which the name Halopiger salifodinae sp. nov. is proposed. The type strain is KCY07-B2(T) ( = JCM 18547(T) = CGMCC 1.12284(T)).
一株从中国新疆库尔勒县盐矿中分离得到的新型极端嗜盐古菌 KCY07-B2(T)。菌落呈奶油色，细胞呈多形杆状。菌株 KCY07-B2(T)能够在 25-50°C（最适 37-45°C）和 pH6.0-8.0（最适 7.0）下生长。该菌株的生长至少需要 1.9M NaCl。不需要 MgCl2。细胞在蒸馏水中溶解。极性脂分析表明存在磷脂酰甘油和磷脂酰甘油磷酸甲基酯，来源于 C20C20 和 C20C25 甘油二醚，以及五种糖脂。存在双硫酸化糖脂 S2-DGD-1。DNA G+C 含量为 62.5mol%。16S rRNA 基因序列分析表明，菌株 KCY07-B2(T)与 Halopiger xanaduensis SH-6(T)和 Halopiger aswanensis 56(T)密切相关（相似度分别为 95.8%和 95.5%）。基于其表型、化学分类学和基因型特征，菌株 KCY07-B2(T)被认为代表了 Halopiger 属的一个新种，命名为 Halopiger salifodinae sp. nov.。模式菌株为 KCY07-B2(T)（=JCM 18547(T)=CGMCC 1.12284(T)）。
