西非葫芦（槽纹南瓜）叶乙醇提取物对α-淀粉酶和α-葡萄糖苷酶活性的抑制作用
Inhibition of α-amylase and α-glucosidase activities by ethanolic extract of Telfairia occidentalis (fluted pumpkin) leaf.
作者信息
Oboh G, Akinyemi A J, Ademiluyi A O
机构信息
Department of Biochemistry,Federal University of Technology, Akure, NigeriaP.M.B., 704, Akure 340001, Nigeria.
出版信息
Asian Pac J Trop Biomed. 2012 Sep;2(9):733-8. doi: 10.1016/S2221-1691(12)60219-6.
Abstract
OBJECTIVE
To investigate the inhibitory effect of Telfairia occidentalis Hook f. (Curcubitaceae) (T. occidentalis) leaf on key enzyme linked to type-2 diabetes (α - amylase and α - glucosidase) as well as assess the effect of blanching (a commonly practiced food processing technique) of the vegetable on these key enzymes.
METHODS
Fresh leaves of T. occidentalis were blanched in hot water for 10 minutes, and the extracts of both the fresh and blanched vegetables were prepared and used for subsequent analysis. The inhibitory effect of the extract on α - amylase and α - glucosidase activities as well as some antioxidant parameter was determined in vitro.
RESULTS
The result revealed that unprocessed T. occidentalis leaf reduce Fe(3+) to Fe(2+) and also inhibited α - amylase and α - glucosidase activities in a dose dependent manner. However, blanching of the leafy vegetables caused a significant (P<0.05) increase in the antioxidant properties but decrease their ability to inhibit α - amylase and α - glucosidase activities.
CONCLUSIONS
This antioxidant properties and enzyme inhibition could be part of the mechanism by which they are used in the treatment/prevention of type-2 diabetes. However, the blanched vegetable reduces their ability to inhibit both α - amylase and α - glucosidase activity in vitro.
摘要
目的
研究西非葫芦（葫芦科）（Telfairia occidentalis Hook f.）叶对与2型糖尿病相关的关键酶（α - 淀粉酶和α - 葡萄糖苷酶）的抑制作用，并评估蔬菜的热烫处理（一种常用的食品加工技术）对这些关键酶的影响。
方法
将新鲜的西非葫芦叶在热水中热烫10分钟，制备新鲜和热烫蔬菜的提取物并用于后续分析。在体外测定提取物对α - 淀粉酶和α - 葡萄糖苷酶活性以及一些抗氧化参数的抑制作用。
结果
结果显示，未加工的西非葫芦叶可将Fe(3+)还原为Fe(2+)，并以剂量依赖性方式抑制α - 淀粉酶和α - 葡萄糖苷酶的活性。然而，叶菜类蔬菜的热烫处理使其抗氧化性能显著（P<0.05）增加，但降低了它们抑制α - 淀粉酶和α - 葡萄糖苷酶活性的能力。
结论
这种抗氧化性能和酶抑制作用可能是它们用于治疗/预防2型糖尿病的机制的一部分。然而，热烫处理的蔬菜在体外降低了它们抑制α - 淀粉酶和α - 葡萄糖苷酶活性的能力。
相似文献
1
Inhibition of α-amylase and α-glucosidase activities by ethanolic extract of Telfairia occidentalis (fluted pumpkin) leaf.西非葫芦（槽纹南瓜）叶乙醇提取物对α-淀粉酶和α-葡萄糖苷酶活性的抑制作用
Asian Pac J Trop Biomed. 2012 Sep;2(9):733-8. doi: 10.1016/S2221-1691(12)60219-6.
2
Antioxidant properties and inhibitory effect of ethanolic extract of Struchium sparganophora (Ewuro Odo) leaf on α--amylase and α--glucosidase activities.散枝猪毛菜（Ewuro Odo）叶乙醇提取物的抗氧化特性及其对α-淀粉酶和α-葡萄糖苷酶活性的抑制作用。
Afr J Tradit Complement Altern Med. 2012 Apr 2;9(3):342-9. doi: 10.4314/ajtcam.v9i3.7. eCollection 2012.
3
Kinetics of α-amylase and α-glucosidase inhibitory potential of Zea mays Linnaeus (Poaceae), Stigma maydis aqueous extract: An in vitro assessment.玉米（禾本科）玉米须水提取物对α-淀粉酶和α-葡萄糖苷酶的抑制动力学：体外评估
J Ethnopharmacol. 2016 May 13;183:1-8. doi: 10.1016/j.jep.2016.02.024. Epub 2016 Feb 21.
4
In vitro inhibition activity of polyphenol-rich extracts from Syzygium aromaticum (L.) Merr. & Perry (Clove) buds against carbohydrate hydrolyzing enzymes linked to type 2 diabetes and Fe(2+)-induced lipid peroxidation in rat pancreas.丁香（Syzygium aromaticum (L.) Merr. & Perry）芽中富含多酚的提取物对与2型糖尿病相关的碳水化合物水解酶的体外抑制活性以及对大鼠胰腺中铁离子（Fe(2+)）诱导的脂质过氧化的影响
Asian Pac J Trop Biomed. 2012 Oct;2(10):774-81. doi: 10.1016/S2221-1691(12)60228-7.
5
-Amylase and -Glucosidase Inhibitory and Antioxidant Activities of the Crude Extract and Solvent Fractions of Leaves.叶片粗提物及溶剂萃取物的α-淀粉酶和α-葡萄糖苷酶抑制活性与抗氧化活性
Biomed Res Int. 2021 Apr 19;2021:6652777. doi: 10.1155/2021/6652777. eCollection 2021.
6
Inhibitory effect of Azadirachta indica A. juss leaf extract on the activities of alpha-amylase and alpha-glucosidase.印楝叶提取物对α-淀粉酶和α-葡萄糖苷酶活性的抑制作用。
Pak J Biol Sci. 2013 Nov 1;16(21):1358-62. doi: 10.3923/pjbs.2013.1358.1362.
7
Inhibitory potentials of phenolic-rich extracts from Bridelia ferruginea on two key carbohydrate-metabolizing enzymes and Fe-induced pancreatic oxidative stress.富含多酚的鹧鸪麻提取物对两种关键糖代谢酶的抑制作用及铁诱导的胰腺氧化应激。
J Integr Med. 2018 May;16(3):192-198. doi: 10.1016/j.joim.2018.04.006. Epub 2018 Apr 18.
8
α-amylase and α-glucosidase Inhibition, Antioxidant, Anti- Inflammatory Activity and GC-MS Profiling of Blume.α-淀粉酶和 α-葡萄糖苷酶抑制、抗氧化、抗炎活性及 GC-MS 分析 Blume。
Comb Chem High Throughput Screen. 2020;23(9):945-954. doi: 10.2174/1386207323666200428081748.
9
Machine Learning and In Vitro Chemical Screening of Potential α-Amylase and α-Glucosidase Inhibitors from Thai Indigenous Plants.机器学习与体外化学筛选泰国本土植物中潜在的α-淀粉酶和α-葡萄糖苷酶抑制剂。
Nutrients. 2022 Jan 9;14(2):267. doi: 10.3390/nu14020267.
10
study on α-amylase and α-glucosidase inhibitory activities of a new stigmastane-type steroid saponin from the leaves of .从. 的叶子中提取的一种新型甾体皂甙的α-淀粉酶和α-葡萄糖苷酶抑制活性研究。
Nat Prod Res. 2021 Mar;35(5):873-879. doi: 10.1080/14786419.2019.1607853. Epub 2019 Jun 17.
引用本文的文献
1
Biosynthesis and characterization of silver nanoparticles from Asplenium dalhousiae and their potential biological properties.利用喜马拉雅铁角蕨合成银纳米颗粒及其表征与潜在生物学特性
PLoS One. 2025 Jun 30;20(6):e0325533. doi: 10.1371/journal.pone.0325533. eCollection 2025.
2
The Influence of Maceration and Flavoring on the Composition and Health-Promoting Properties of Roasted Coffee.浸提和调味对烘焙咖啡成分和促进健康特性的影响。
Nutrients. 2024 Aug 23;16(17):2823. doi: 10.3390/nu16172823.
3
Fermentation of Rubus dolichocarpus juice using Lactobacillus gasseri and Lacticaseibacillus casei and protecting phenolic compounds by Stevia extract during cold storage.利用格氏乳杆菌和干酪乳杆菌发酵树莓汁，并在冷藏过程中用甜菊提取物保护酚类化合物。
Sci Rep. 2024 Mar 8;14(1):5711. doi: 10.1038/s41598-024-56235-7.
4
L. Aqueous and Ethyl Acetate Extracts: Antioxidant Effect and Potential Activity In Vitro and In Vivo against Pancreatic α-Amylase and Intestinal α-Glucosidase.L. 水提取物和乙酸乙酯提取物：体外和体内对胰腺α-淀粉酶和肠道α-葡萄糖苷酶的抗氧化作用及潜在活性。
Pharmaceutics. 2022 Feb 22;14(3):481. doi: 10.3390/pharmaceutics14030481.
5
Inhibition of the Activities of α-Amylase and Pancreatic Lipase by Aqueous Extracts of and Leaves.**和**叶水提取物对α-淀粉酶和胰脂肪酶活性的抑制作用。
需注意，原文中“和”处内容缺失，请补充完整以便准确理解和更完善地翻译。
Front Nutr. 2021 Nov 8;8:772903. doi: 10.3389/fnut.2021.772903. eCollection 2021.
6
Antagonistic, Anti-oxidant, Anti-inflammatory and Anti-diabetic Probiotic Potential of Isolated From the Rhizosphere of the Medicinal Plants.从药用植物根际分离的益生菌的拮抗、抗氧化、抗炎和抗糖尿病潜力
Saudi J Biol Sci. 2021 Nov;28(11):6069-6076. doi: 10.1016/j.sjbs.2021.08.029. Epub 2021 Aug 18.
7
Leaves Extracts: In Vitro Carbohydrate Digestive Enzymes Inhibition and Phytochemical Characterization.叶片提取物：体外碳水化合物消化酶抑制作用及植物化学成分分析。
Molecules. 2021 Oct 15;26(20):6229. doi: 10.3390/molecules26206229.
8
Nutritional, Phytochemical Characteristics and In Vitro Effect on α-Amylase, α-Glucosidase, Lipase, and Cholinesterase Activities of 12 Coloured Carrot Varieties.12个彩色胡萝卜品种的营养、植物化学特性及其对α-淀粉酶、α-葡萄糖苷酶、脂肪酶和胆碱酯酶活性的体外影响
Foods. 2021 Apr 9;10(4):808. doi: 10.3390/foods10040808.
9
Inhibitory Activities of Polyphenolic Extracts of Bangladeshi Vegetables against α-Amylase, α-Glucosidase, Pancreatic Lipase, Renin, and Angiotensin-Converting Enzyme.孟加拉国蔬菜多酚提取物对α-淀粉酶、α-葡萄糖苷酶、胰脂肪酶、肾素和血管紧张素转换酶的抑制活性。
Foods. 2020 Jun 29;9(7):844. doi: 10.3390/foods9070844.
10
Polyphenolic compositions and angiotensin-I-converting enzyme inhibitory properties of common green leafy vegetables: A comparative study.常见绿叶蔬菜的多酚成分及血管紧张素I转换酶抑制特性：一项比较研究。
Food Sci Biotechnol. 2016 Oct 31;25(5):1243-1249. doi: 10.1007/s10068-016-0197-1. eCollection 2016.
本文引用的文献
1
Inhibitory effect of some tropical green leafy vegetables on key enzymes linked to Alzheimer's disease and some pro-oxidant induced lipid peroxidation in rats' brain.一些热带绿叶蔬菜对与阿尔茨海默病相关的关键酶的抑制作用及对大鼠脑组织中一些促氧化剂诱导的脂质过氧化的抑制作用。
J Food Sci Technol. 2014 May;51(5):884-91. doi: 10.1007/s13197-011-0572-0. Epub 2011 Nov 4.
2
Chemistry and biochemistry of dietary polyphenols.膳食多酚的化学与生物化学。
Nutrients. 2010 Dec;2(12):1231-46. doi: 10.3390/nu2121231. Epub 2010 Dec 10.
3
α-glucosidase inhibitors from plants: A natural approach to treat diabetes.植物来源的α-葡萄糖苷酶抑制剂：一种治疗糖尿病的天然方法。
Pharmacogn Rev. 2011 Jan;5(9):19-29. doi: 10.4103/0973-7847.79096.
4
Pharmacological management of type 2 diabetes mellitus: an update.2型糖尿病的药物治疗：最新进展
Curr Diabetes Rev. 2011 Nov;7(6):392-405. doi: 10.2174/157339911797579160.
5
Effect of combination on the antioxidant and inhibitory properties of tropical pepper varieties against α-amylase and α-glucosidase activities in vitro.组合对热带胡椒品种体外抗氧化和抑制α-淀粉酶和α-葡萄糖苷酶活性的影响。
J Med Food. 2011 Oct;14(10):1152-8. doi: 10.1089/jmf.2010.0194. Epub 2011 Jun 11.
6
Oxidative stress and the etiology of insulin resistance and type 2 diabetes.氧化应激与胰岛素抵抗和 2 型糖尿病的病因。
Free Radic Biol Med. 2011 Sep 1;51(5):993-9. doi: 10.1016/j.freeradbiomed.2010.12.005. Epub 2010 Dec 13.
7
Cinnamon and health.肉桂与健康。
Crit Rev Food Sci Nutr. 2010 Oct;50(9):822-34. doi: 10.1080/10408390902773052.
8
Phenolic compounds, antioxidant activity and in vitro inhibitory potential against key enzymes relevant for hyperglycemia and hypertension of commonly used medicinal plants, herbs and spices in Latin America.拉丁美洲常用药用植物、草药和香料中的酚类化合物、抗氧化活性及体外抑制高血糖和高血压相关关键酶的潜力
Bioresour Technol. 2010 Jun;101(12):4676-89. doi: 10.1016/j.biortech.2010.01.093. Epub 2010 Feb 25.
9
Inhibition of cyclophosphamide-induced oxidative stress in rat brain by polar and non-polar extracts of Annatto (Bixa orellana) seeds.红木种子的极性和非极性提取物对环磷酰胺诱导的大鼠脑氧化应激的抑制作用。
Exp Toxicol Pathol. 2011 Mar;63(3):257-62. doi: 10.1016/j.etp.2010.01.003.
10
Phenolic-linked variation in strawberry cultivars for potential dietary management of hyperglycemia and related complications of hypertension.草莓品种中酚类物质相关变异对高血糖及高血压相关并发症的潜在膳食管理作用
Bioresour Technol. 2010 Jan;101(1):404-13. doi: 10.1016/j.biortech.2009.07.068. Epub 2009 Aug 19.
Zotero 插件