单细胞中金属检测技术。

Technologies for detecting metals in single cells.

作者信息

Penner-Hahn James E

机构信息

Departments of Chemistry and Biophysics, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109-1055, USA,

出版信息

Met Ions Life Sci. 2013;12:15-40. doi: 10.1007/978-94-007-5561-1_2.

DOI:10.1007/978-94-007-5561-1_2

PMID:23595669

Abstract

In order to fully understand the metallomics of an organism, it is essential to know how much metal is present in each cell and, ideally, to know both the spatial and chemical distributions of each metal (i.e., where within the cell is a metal found, and in what chemical form). No single technique provides all of this information. This chapter reviews the various methods that can be used and the strengths and weaknesses of each.

摘要

为了全面了解生物体的金属组学，必须知道每个细胞中存在多少金属，理想情况下，还需知道每种金属的空间分布和化学分布（即细胞内何处发现金属以及以何种化学形式存在）。没有单一技术能提供所有这些信息。本章综述了可使用的各种方法以及每种方法的优缺点。