Mermans Fabian, Mattelin Valérie, Van den Eeckhoudt Ruben, García-Timermans Cristina, Van Landuyt Josefien, Guo Yuting, Taurino Irene, Tavernier Filip, Kraft Michael, Khan Hira, Boon Nico
Center for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University, Ghent, Belgium.
Department of Oral Health Sciences, KU Leuven, Leuven, Belgium.
Front Microbiol. 2023 Aug 25;14:1233705. doi: 10.3389/fmicb.2023.1233705. eCollection 2023.
New techniques are revolutionizing single-cell research, allowing us to study microbes at unprecedented scales and in unparalleled depth. This review highlights the state-of-the-art technologies in single-cell analysis in microbial ecology applications, with particular attention to both optical tools, i.e., specialized use of flow cytometry and Raman spectroscopy and emerging electrical techniques. The objectives of this review include showcasing the diversity of single-cell optical approaches for studying microbiological phenomena, highlighting successful applications in understanding microbial systems, discussing emerging techniques, and encouraging the combination of established and novel approaches to address research questions. The review aims to answer key questions such as how single-cell approaches have advanced our understanding of individual and interacting cells, how they have been used to study uncultured microbes, which new analysis tools will become widespread, and how they contribute to our knowledge of ecological interactions.
新技术正在彻底改变单细胞研究，使我们能够以前所未有的规模和无与伦比的深度研究微生物。本综述重点介绍了微生物生态学应用中单细胞分析的前沿技术，特别关注光学工具，即流式细胞术和拉曼光谱的专门应用以及新兴的电学技术。本综述的目的包括展示用于研究微生物现象的单细胞光学方法的多样性，突出在理解微生物系统方面的成功应用，讨论新兴技术，并鼓励结合成熟方法和新方法来解决研究问题。该综述旨在回答一些关键问题，例如单细胞方法如何推进我们对单个细胞和相互作用细胞的理解，它们如何被用于研究未培养的微生物，哪些新的分析工具将得到广泛应用，以及它们如何增进我们对生态相互作用的认识。
