Department of Biomedical Engineering, Biomedical NMR, Eindhoven University of Technology, Eindhoven, The Netherlands.
J Control Release. 2013 Jul 10;169(1-2):82-90. doi: 10.1016/j.jconrel.2013.04.005. Epub 2013 Apr 15.
The goal of this study was to investigate the blood kinetics and biodistribution of temperature-sensitive liposomes (TSLs) for MR image-guided drug delivery. The co-encapsulated doxorubicin and [Gd(HPDO3A)(H₂O)] as well as the ¹¹¹In-labeled liposomal carrier were quantified in blood and organs of tumor bearing rats. After TSL injection, mild hyperthermia (T=42 °C) was induced in the tumor using high intensity focused ultrasound under MR image-guidance (MR-HIFU). The biodistribution of the radiolabeled TSLs was investigated using SPECT/CT imaging, where the highest uptake of ¹¹¹In-labeled TSLs was observed in the spleen and liver. The MR-HIFU-treated tumors showed 4.4 times higher liposome uptake after 48 h in comparison with controls, while the doxorubicin concentration was increased by a factor of 7.9. These effects of HIFU-treatment are promising for applications in liposomal drug delivery to tumors.
本研究旨在研究温度敏感脂质体（TSL）的血液动力学和生物分布，用于磁共振成像引导的药物输送。阿霉素和 [Gd(HPDO3A)(H₂O)] 共包封物以及 ¹¹¹In 标记的脂质体载体在荷瘤大鼠的血液和器官中进行定量分析。在 TSL 注射后，使用高强度聚焦超声（MR 图像引导下的 MR-HIFU）在肿瘤中诱导温和的热疗（T=42°C）。使用 SPECT/CT 成像研究放射性标记的 TSL 的生物分布，其中¹¹¹In 标记的 TSL 在脾脏和肝脏中的摄取最高。与对照组相比，MR-HIFU 治疗的肿瘤在 48 小时后摄取的脂质体增加了 4.4 倍，而阿霉素的浓度增加了 7.9 倍。这些 HIFU 治疗的效果为脂质体药物输送到肿瘤的应用提供了希望。
