Hussain Arshad, Shadma Wahab, Maksood Ali, Ansari Shahid Hussain
Department of Pharmacy, Integral University, Kursi road, Dasauli, Lucknow, Uttar Pradesh, India.
Pharmacognosy Res. 2013 Jan;5(1):30-5. doi: 10.4103/0974-8490.105646.
To study the immunomodulatory effect of ethanolic and aqueous extract of the rhizomes of Picrorrhiza kurroa (Scrophulariaceae) in normal and immunosuppressed mice models.
The rhizomes extract of Picrorrhiza kurroa was administered orally according to their body weight in mice. The study was carried out by various hematological and serological tests. The assessment of immunomodulatory activity on specific and non-specific immunity was studied by administration of test extract. The method of cyclophasphamide-induced immunosuppression was employed with slight modification to study the immunomodulatory potential of the extract. Plant extracts were administered by oral feeding canula to the test groups (groups III-VI), group I (control animals) and group II (model control animals) received same volume of normal saline (0.2 ml). Humoral antibody response to SRBC measurement of antibody titer by hemagglutination reaction was done. The mice belonging to the all groups were antigenically challenged with SRBC (0.5×10(9) cells/ml/100 g) on 10(th) day intraparitoneally. Cellular immune response (Foot pad reaction test) the edema was induced by injecting SRBC (0.025×10(9) cells) in left paw, and 0.025 ml of saline was injected in right paw.
The plant extract showed protective effects on humoral immunity. The change in percentage deduction in footpad volume was also found significant (P<0.001). Administration of extract remarkably ameliorated both cellular and humoral antibody response.
It is concluded that the test extracts possessed promising immunostimulant properties. But, the alcoholic extract is more potent than aqueous extract in producing delayed type hypersensitivity response.
研究胡黄连（玄参科）根茎乙醇提取物和水提取物对正常及免疫抑制小鼠模型的免疫调节作用。
根据小鼠体重口服给予胡黄连根茎提取物。通过各种血液学和血清学检测进行研究。通过给予受试提取物研究其对特异性和非特异性免疫的免疫调节活性评估。采用环磷酰胺诱导免疫抑制的方法并稍作修改来研究提取物的免疫调节潜力。通过口服灌胃将植物提取物给予试验组（III - VI组），I组（对照动物）和II组（模型对照动物）给予相同体积的生理盐水（0.2 ml）。通过血凝反应测定对SRBC的体液抗体反应，检测抗体效价。在第10天腹腔内用SRBC（0.5×10⁹个细胞/ml/100 g）对所有组的小鼠进行抗原攻击。细胞免疫反应（足垫反应试验）：在左爪注射SRBC（0.025×10⁹个细胞）诱导水肿，右爪注射0.025 ml生理盐水。
植物提取物对体液免疫显示出保护作用。足垫体积减少百分比的变化也具有显著性（P<0.001）。提取物的给予显著改善了细胞和体液抗体反应。
得出受试提取物具有良好免疫刺激特性的结论。但是，乙醇提取物在产生迟发型超敏反应方面比水提取物更有效。
