Raj Sreena, Gothandam K M
School of Bio Sciences and Technology, VIT University, Vellore 632014, India.
School of Bio Sciences and Technology, VIT University, Vellore 632014, India.
Food Chem Toxicol. 2015 Jul;81:151-159. doi: 10.1016/j.fct.2015.04.026. Epub 2015 Apr 24.
The present study investigates the immunomodulatory activity of methanolic extract of Amorphophallus commutatus var. wayanadensis (MEAC) under normal and cyclophosphamide induced immunosuppressive conditions in Swiss albino mice models. The splenocyte proliferation assay was performed to study in-vitro immunomodulatory activity of MEAC, where sheep RBC (SRBC) was used to induce immune responses in the experimental animals. The in-vivo immunomodulatory activity was evaluated by humoral antibody titer, quantification of plaque forming cells, qualitative hemolysis, delayed type hypersensitivity assay, phagocytic index and neutrophil adhesion assays. The chemoprotective effect of MEAC was determined against cyclophosphamide induced immunosuppression in mice models. MEAC exhibited significant mitogenic and co-mitogenic activity on Con-A, PHA and LPS stimulated splenocytes isolated from mouse spleen in a dose dependent manner. Furthermore, MEAC also elicited significant immunomodulatory activity with enhanced activation of humoral immune response along with a suppressive effect on cell mediated immune response. Hematological and histopathological analysis revealed the protective effect of MEAC against CP induced immunosuppression. The significant immunomodulatory activity of MEAC observed in the current study could be due to the fatty acids and phytosterols present in the extract.
本研究在瑞士白化小鼠模型中，研究了疣柄魔芋瓦亚纳德变种甲醇提取物（MEAC）在正常和环磷酰胺诱导的免疫抑制条件下的免疫调节活性。进行脾细胞增殖试验以研究MEAC的体外免疫调节活性，其中使用绵羊红细胞（SRBC）在实验动物中诱导免疫反应。通过体液抗体滴度、斑块形成细胞定量、定性溶血、迟发型超敏反应试验、吞噬指数和中性粒细胞黏附试验评估体内免疫调节活性。确定了MEAC对小鼠模型中环磷酰胺诱导的免疫抑制的化学保护作用。MEAC对从小鼠脾脏分离的Con-A、PHA和LPS刺激的脾细胞表现出显著的促有丝分裂和共促有丝分裂活性，且呈剂量依赖性。此外，MEAC还引发了显著的免疫调节活性，增强了体液免疫反应的激活，并对细胞介导的免疫反应具有抑制作用。血液学和组织病理学分析揭示了MEAC对环磷酰胺诱导的免疫抑制的保护作用。在本研究中观察到的MEAC显著的免疫调节活性可能归因于提取物中存在的脂肪酸和植物甾醇。
